Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. In the registers below you will find more information about the different application areas and suitable product recommendations for optimal cultivation.
Alternative application areas such as mixing or thawing can be found in the second section of this page.
Stem cell cultivations run for several days or weeks and normally requires specific cultivation conditions, such as a low oxygen concentration (1-8%), high carbon dioxide concentration (4-10%), and a high relative humidity (>70%). Stem cells are mostly adherent cultures and are very sensitive to shear stress. Orbital shaken bioreactors are ideally suited for stem cell cultivations since they have a low local power input, therefore, significantly reducing the mechanical shear stress on the cultivated stem cells.
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Cultivations of animal or human cell cultures run for several days or a few weeks. Usually, for cultivations they require a high carbon dioxide concentration (4-10%) and a high relative humidity (>70%). Cell cultures grow adherent or in suspension. Moreover, temperature shifts from 37 °C to 32 °C are often conducted during cultivations. When cultivating at a high relative humidity, this requires active dehumidifying of the incubator air to prevent condensation at the door or walls of the incubator shaker.
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Cultivations of yeasts and fungi run for approximately 3 to 5 days. Yeasts
and fungi have a higher oxygen demand than animal or human cell cultures. A
higher oxygen transfer can be achieved by decreasing the filling volume and
increasing the shaking frequency. Moreover, active humidity control might be
helpful to reduce evaporation losses. Filamentous fungi can increase the
viscosity of the cultivation medium. In that case, large shaking diameters (50
or even 70 mm) should be chosen to prevent the occurrence of the “out-of-phase”
phenomenon.
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Increasing the shaking capacity: up to three ISF1-Z incubator shakers or up to two LT-X incubator shakers can be easily stacked without any additional accessories (stacked incubator shakers can also be operated at high shaking frequencies).
Increasing the shaking capacity for identical cultivation conditions: ISF4-X – it allows for up to five individual shaking tables in one incubator chamber.
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Cultivations of bacteria run for several hours or days. Bacteria usually have a much higher oxygen demand than animal or human cell cultures and often also a higher oxygen demand than yeasts or fungi. A high oxygen transfer can be achieved by small filling volumes and high shaking frequencies (300-350 rpm). Moreover, a humidity control is helpful to prevent high evaporation losses (especially for very small filling volumes and cultivation temperatures of 30-37 °C). For very small filling volumes, large shaking diameters (50 or even 70 mm) should be chosen to prevent the occurrence of the “out-of-phase” phenomenon.
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Cultivations of plant cell cultures and algae run for approximately 5 to 7 days. The oxygen demand lies in between animal cell cultures and bacteria. For longer cultivations, active humidity control is useful to decrease evaporation from the cultivation vessels. Many cultures require LED light for their photosynthesis reaction. Also, depending on the plant cell culture, CO2 control might be necessary.
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Shaker with LED light module and CO2 control:
Two options are available for integrating the LED light module:
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Shaker with LED light module and CO2 control:
Two options are available for integrating the LED light module:
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In many cases, different types of liquids must be thawed gently and evenly in a laboratory besides the cultivation of cell cultures and microorganisms. These liquids include for instance cultivation media, cell cultures, protein and enzyme solutions.
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In many cases, different kinds of liquids must be mixed or shaken in a laboratory besides the cultivation of cell cultures and microorganisms. These liquids may include chemical substances, oils with particulate matter, liquid/crystalline mixtures, protein, enzyme solutions, blood bags, or solutions containing catalysts.
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