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The Kuhner TOM (Transfer-Rate Online Measurement)
provides online oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR),
and respiratory quotient (RQ), allowing for data driven decision making using
high value physiological data.
Reliable and reproducible data is provided from a
fully automated setup, 24/7 without additional manual work. Simply connect your
existing shake flask and minimize downtime and costs for extra consumables.
The Kuhner TOM provides the user with a better
understanding of any bioprocess and allows for more efficient and effective
optimization during process development. It is easily installed in any Kuhner
shaker and is compatible with a variety of different shake flask types and
sizes, including single use shake flasks.
The Kuhner TOM is now available with an extended
temperature range of up to
58°C option. Cultivation temperatures above 40°C are typically required for
growing thermophilic organisms or for biodegradation processes. This option is
also retrofittable to existing Kuhner TOMs.
Online Measurement
Flexibility
Application
Retrofittable
The Kuhner TOM M is specifically optimized for microbial cultivation, ensuring reliable data collection and process conditions in non-CO₂ controlled pressurized air connections allows for strong aeration, even for large flask and high oxygen demand.
The Kuhner TOM C is optimized for cell culture applications, delivering precise data collection in humidified and CO₂-controlled incubators. It uses the air from the incubator to aerate the shake flasks, no further equipment necessary and maximum comparability to your existing setup.
The modularity of the Kuhner TOM allows the user to choose online measure-ments from 4, 8, 12, or 16 shake flasks. The Kuhner TOM itself is mounted above the shake flasks and requires no space on the shaking platform, maximizing the capacity for shake flasks in the incubator. Kuhner TOM can easily be topped up later. Start with 4 flasks and add another module whenever your demand is growing.
Reduce your sampling expense drastically
The Kuhner TOM captures the progress of multiple cultivations over time, providing an opportunity for the user to selectively schedule off-line sampling and analysis at critical time points. The Kuhner TOM offers valuable insight on the viability and growth rate of shaken cultivations and will also help identify sub-optimal conditions, including oxygen limitation, substrate limitation, or product inhibition. The Kuhner TOM also provides an opportunity for the systematic and rapid optimization of culture media and conditions (e.g. balancing different carbon sources or media additives, substrate uptake and product formation via RQ). The OTR, CTR, and RQ data captured from the Kuhner TOM will accelerate any process development and facilitates scale-up.
You can rent a Kuhner TOM system directly from your local distributor.
Rental systems are for temporary use and must be returned at the end of the trial period.
Temperature range | 15-40°C (58°C on request) |
Shaking speed | < 600 rpm |
Orbital shaking diameter | 3 mm – 70 mm |
Flask size |
250 mL – 1000 mL (smaller and larger
flasks on request) |
Filling volume | > 4% of nominal flask volume |
Humidity | < 85% rel. humidity |
Concentration range | CO2 < 16% |
Maintenance | CO2 sensor calibration once per month Pump adjustment after 1 year |
Warranty time | 2 years |
Technical data subject to change | |
The FlexCap is a universal silicone cap that allows the use of different sized shake flasks in the Kuhner TOM system for measuring oxygen and carbon dioxide transfer rates. Its special design makes it possible to seal both small and narrow flasks (min. 25 mm inner neck diameter) and large and wide flasks (max. 55 mm outer neck diameter) with the same sealing cap. The FlexCap also makes cultivations in special flasks and shake flasks with baffles possible for online measurements with the Kuhner TOM system.
In addition, the FlexCap can also be used for aeration and humidification of shake flasks with FlowCon and Kuhner AerationSystem (consisting of FlexCaps and GasDivider). This enables the user to successfully carry out demanding and sensitive cell culture processes even in shaking incubators without humidity and O2/CO2 control. The FlowCon provides precise gas composition and flow rate, while the GasDivider humidifies the gas flow and distributes it evenly to the inlet ports of the FlexCaps. In addition to the connections for the air inlet and outlet, the FlexCap has an additional connection for substrate addition and sampling. The FlexCap is reusable and can be sterilised by autoclaving at 121 °C.
Compatibility:
100 mL to 1 L wide-mouth glass Erlenmeyer flasks
250 mL to 5 L narrow-mouth glass Erlenmeyer flasks
GL45 flasks with baffles at the bottom (provided conditions are splash-free)
Thomson Ultrayield Kolben 250, 500, 1000 mL
Corning 250 mL flasks and 50 mL tube
Nalgene
250 mL flasks
Order number | Description |
|---|---|
107105 | Kuhner FlexCap |
Führen Sie biologische Kultivierungen in einem besonders grossen, selbstentwickelten oder unkonventionellen Schüttelgefäss durch? Und hätten Sie gerne Online-Monitoring-Daten dieser Kultivierungen, um mehr Informationen über Ihren Bioprozesses zu erhalten?
Kühner bietet massgeschneiderte Verschlusskappen für alle Arten von Schüttelgefässen an, um diese schnell und einfach an das Kuhner TOM System anschliessen zu können. Dies bietet die Möglichkeit, Online-Messungen von Atmungs- und Stoffwechselaktivität für mikrobielle Kulturen und Zellkulturen auch in diesen Kultivierungsgefäßen durchzuführen.
Kühner hat beispielsweise eine Kuhner TOM Verschlusskappe für Thomson Optimum Growth Kolben entwickelt, welche routinemässig für Zellkulturprozesse eingesetzt werden. Diese Spezialkappe passt auf 1.6L, 2.8L und 5L-Thomson Optimum Growth Kolben. Die Thomson Optimum Growth-Cap ist wiederverwendbar und sterilisierbar durch Autoklavieren bei 121 °C.
Wenn Sie Interesse an der Thomson-Cap oder an einer kundenspezifischen Verschlusskappe für Ihr eigenes Kultivierungsgefäß haben, kontaktieren Sie uns gerne: office@kuhner.com
| Order number | Description | Compatibility |
|---|---|---|
| 102443 | GL45 screw cap | Kuhner TOM Kolben |
| 104347 | Cell culture adapter lid | Various single use pistons and tubes |
| 102863 | Adapter Ring 50mL tube | Corning, TPP 50mL Tubes |
| 104352 | Adapter Ring 125mL | Corning 125mL PC flask |
| 104253 | Adapter Ring 250mL | Corning 250mL PC flask |
| 104354 | Adapter Ring 500/1000mL | Corning 500/1000mL PC flask |
For use with the Kuhner TOM, we offer a selection of specially developed Kuhner TOM flasks with a volume of 250 mL, 500 mL and 1000 mL. All flasks are fitted with a GL45 screw cap to ensure a secure seal and connection to the Kuhner TOM. Shake flasks with 2000mL, 5000mL or with special geometry are also available on request.
Order number | Description |
|---|---|
102784 | Kuhner TOM flasks 250 mL |
102787 | Kuhner TOM flasks 500 mL |
102789 | Kuhner TOM flasks 1000 mL |
On request | Kuhner TOM flasks 2000 mL |
On request | Kuhner TOM flasks 5000 mL |
On request | Special geometry flasks |
To ensure seamless integration of the Kuhner TOM into various incubator models, a range of installation kits is available. These kits enable safe and stable installation in incubators such as the ISF1-X, LT-X, ISF1-Z, Kuhner Kelvin+ and ISF4-X. Each kit is customised to the respective incubator to ensure optimal positioning and function. In addition, a mounting option is available where the Kuhner TOM is mounted on the side wall of the incubator - ideal for large flasks or photosynthesis applications.
| Order number | Description | Compatibility |
|---|---|---|
| 104380 | Kuhner TOM installation kit in ISF1-X | ISF1-X Incubator Shaker |
| 104382 | Kuhner TOM installation kit in LT-X | LT-X Incubator Shaker |
| 104384 | Kuhner TOM installation kit in ISF1-Z | ISF1-Z Incubator Shaker |
| 104403 | Kuhner TOM installation kit in Kelvin+ | Kuhner Kelvin+ Incubator Shaker |
| On request | Installation kit Kuhner TOM in ISF4-X | ISF4-X Incubator Shaker |
| On request | Side Wall installation kit | All incubators, max. 2nd module, for large flasks or photosynthesis |
We recommend the use of our flask clamps with anti-slip coating. These ensure that the shake flasks cannot rotate during cultivation and that the connection to the Kuhner TOM is not interrupted. Kuhner TOM must be permanently connected to a PC or laptop. This controls the Kuhner TOM and displays the measured variables. Our laboratory computer is designed for 24/7 operation. The closed housing with passive cooling system makes it extremely quiet and well protected against dust and liquids.
| Order number | Description | Compatibility |
|---|---|---|
| 102814 | Clamps 250mL | 250 mL glass and synthetic flasks |
| 102823 | Clamps 500mL | 500 mL glass and synthetic flasks |
| 102824 | Clamps 1000mL | 1000 mL glass and synthetic flasks |
| 102809 | Measuring computer |
To ensure the long-term reliability and functionality of the Kuhner TOM system, a complete range of spare parts is available.
Order number | Description | Compatibility |
|---|---|---|
102624 | Male
connectors set | Flexcap, GL45, Screw cap and cell culture adapter |
109782 | Male connectors set HT | High temperature connection for Flexcap, GL45, Screw cap and cell culture adapter |
102628 | Calibration connectors | Connectors for CO2 sensor calibration |
109781 | Calibration connectors HT | High temperature connectors for CO2 sensor calibration |
108766 | RS: Maintenance kit | 2m tubing + 20 O-Rings for connectors |
106237 | Kuhner TOM Filter set | Kuhner TOM GL 45 ScrewCap |
102511 | Filter Slim | FlexCap |
102343 | Kuhner TOM O2 Sensor | Replacement O2 sensor |
107341 | RS: Kuhner TOM CO2 Sensor | Replacement CO2 sensor |
109836 | RS: Kuhner TOM Valve | Replacement valve |
Cultivation of entomopathogenic fungi in orbitally shaken bioreactors
Kuhner TOM for off-gas analysis in shake flasks
Kuhner TOM system for analyzing the respiratory activity of a CHO cell pool expressing SARS-CoV-2 Spike Protein
Kuhner TOM system for analyzing the respiratory activity of a CHO cell pool expressing SARS-CoV-2 Spike Protein in a Fed-Batch process
Examining the respiratory activity of CHO cell pool expressing SARS-CoV-2 spike protein across shake flask scales using the Kuhner TOM system
Off-gas analysis of Komagataella phaffii on different carbon sources
Impact of protein production on metabolic activity of CHO stable cell line producing pali****mab
Effect on elevated carbon dioxide concentration on IgG production using CHL-YN cells
Online EtOH measurement in shake flask
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
Kuhner TOM - Transfer-Rate Online Measurement
The Kuhner TOM (Transfer-Rate Online Measurement) provides online measurements of the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) for up to 16 individual shake flasks in a Kuhner incubator. The Kuhner TOM provides the user with a better understanding of any bioprocess and allows for more efficient and effective optimization during process development. It is easily installed in any Kuhner shaker and is compatible with a variety of different shake flask types and sizes, including single use shake flasks.
The following flyers provide information about the machines and function-specific solutions.
Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.
Adolf Kühner AG (Headquarters)
Dinkelbergstrasse 1
CH-4127 Birsfelden (Basel)
Switzerland
We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
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The world's most reliable shaking machines built in-house in Switzerland.
