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Humidification and a controlled gas composition for carrying out cell cultivations

Do you have a shaking incubator with a small capacity that is restricted to temperature control? Would you like to implement humidification and a controlled gas composition for carrying out cell cultivations?

The combination of FlowCon and AerationSystem from Kuhner enables the user to run shake flasks cultivations in non-humidified and non-CO2-controlled incubator shakers. The FlowCon provides a precise gas composition and flow rate.  Simultaneously, the AerationSystem (consisting of GasDivider and AerationCaps) humidifies and distributes the gas flow evenly to the connected shake flasks, preventing high evaporation losses and stabilizes the pH of the media. By controlling the chemical composition of the inlet air with the FlowCon, it is not only possible to supplement CO2 but also to reduce the concentration of oxygen (O2) or even implement hypoxic conditions using nitrogen (N2)*. This control of gas compositions allows for a variety of different applications, including stem cell processes that require physiological oxygen concentration at 5% O2 or anaerobic cultivations in a nitrogen atmosphere.

Humidity control


CO2 control







The Kuhner FlowCon is a stand-alone gas mixing device, which has been developed for the R&D environment and the initial production steps in bioreactors with disposable bags. It provides the perfect headspace composition for your cultures by mixing up to four gases (including both pure and mixed gases). Whether stabilizing the pH in cell cultivations with CO2, or reducing oxygen concentration for microaerophilic organisms, the FlowCon gas mixer meets all of the user’s needs. The FlowCon can be used as a stand-alone device and is also fully compatible with other Kuhner equipment (including shaking incubators, disposable bioreactors, etc.).




The Kuhner FlowCon provides a single mixed and dry gas flow, which usually requires humidification and even distribution across several shake flasks. This task is performed by the Kuhner GasDivider which humidifies the gas flow and provides a precise gas distribution independent of the flask type attached, the tube length or any filters in-between. As the GasDivider is placed on the shaking tray inside the incubator, the gas flow is also perfectly adapted to the incubator temperature.

Shake flask caps: AerationCaps and FlexCaps

Nowadays, numerous shake flask variants are used in laboratories. To counter this diversity, Kuhner offers two different closure caps for the AerationSystem: AerationCaps and FlexCaps.

The Kuhner AerationCaps are used for aeration of standard single-use shake flasks (e.g., corning). They can be quickly and easily attached to the sterile caps ("Vent Caps"). Each AerationCap has two identical nozzles that are used for supply and exhaust air. The AerationCaps (as well as the FlexCaps) are connected to the GasDivider via gas-tight tubes. The AerationCaps are reusable, but not autoclavable. Since they are placed on the sterile covers of the single-use shake flasks, they do not come into contact with culture liquid and therefore do not need to be sterilized.


The Kuhner FlexCap is a universal silicone adapter lid that enables the utilization of various sizes and forms of shake flasks with the Kuhner AerationSystem. Its specific construction seals both the smaller and more narrow flasks (min. 25 mm inner neck diameter) and the larger and wider flasks (max. 55 mm outer neck diameter), respectively, using the same universal lid. With the FlexCap, also cultivations in custom-made flasks and baffled shake flasks are accessible for aeration and humidification with the AerationSystem. Besides the ports for supply and exhaust air, the FlexCap comes with an extra port for external feeding and sampling. Best of all, the FlexCap is reusable by autoclaving at temperatures up to 121°C without losing its mechanical performance.


Mammalian Cell Cultivation using AerationCaps for Shake Flask Gas Supply

In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.

Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.

Stem cells
Cell culture
Plant cells & algae


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