Select a different language or country,
to view content for your location.

SB10-X single-use bioreactor

SB10-X single-use bioreactor
Technical Data

Orbital shaken benchtop bioreactor for single-use bags

The SB10-X is an orbital shaken benchtop bioreactor system for the cultivation of human, mammalian and plant cells in a single-use bag. It consists of six modules (excluding the bag) and has a working volume from 4 litres up to 12 litres.
The SB10-X can be used in research, process development or as a pilot scale bioreactor.

Performance characteristics

  • Cultivation of human, mammalian and plant cells

  • Fast, simple set up

  • Easy scale-up

  • Online measurement and control of pH and DO

  • Three different types of single-use bags available

5 year warranty

5 year warranty







Shaking diameter

Dr. Shakers Helpful Hint N°4 explains what the shaking diameter is and how you can determine the shaking diameter of your lab shaker.

Find information on the importance of the shaking diameter for biological cultivations in our Shaking Technology Forum: Why is the shaking diameter important?

Dr. Shaker


Single-use bag for a fast and simple set up

The cylindrical vessel accepts a single-use 3D bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The standard bag has built-in optical sensors for pH/DO allowing online measurement and control of pH and DO in the medium. Two other bag types are available: a basic single-use bag with no optical sensors and a perfusion bag for ATF and TFF perfusion.

Orbital motion

Orbital motion

The orbital motion of the SB10-X ensures efficient liquid mixing. It facilitates high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB10-X uses the unique Kuhner direct drive which is very robust and provides accurate control of the orbital speed.


Trouble free scale-up

The SB10-X fills a gap in the scale-up chain from commonly used single-use flasks all the way up to 50 L and 200 L bioreactors. The consistent hydrodynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range. The culture from the SB10-X can serve as a preculture for the SB50-X and SB200-X. The SB10-X can also be used as a pilot scale bioreactor.

Online measurement

Heating and online pH & DO measurement

The tray module, which is fixed onto the ES-X shaker module, contains integrated heating and the connections for online pH and DO measurement.


Vessel module

Vessel module


Special emphasis has been placed on developing a user-friendly vessel module. The vessel together with its bag can be easily detached by the user and carried to the clean bench.

For the SB10-X, there are two vessel modules available with different working volumes. The standard module is a 10L-vessel module with a working volume of 4 to 12 L. The 3L-vessel module has a working volume of 1.5 to 4.5 L and is either available as an option in addition to the standard SB10-X or customers may purchase the SB10-X with the 3L-vessel only.

Filter heater

Filter heater

A filter heater for one exhaust filter is placed above the vessel module. The magnetic mounting makes handling the filter heater very simple and allows its location in various places. The filter heater combats water vapour condensation in the outlet air preventing blockages in the membrane


Control unit

Control unit and software


The compact and functional design of the control unit provides easy handling and monitoring of the bioprocess parameters. The control unit has an integrated and adjustable 12” touch screen with USB and Ethernet connections. The Kuhner Insight application software for data recording, calibration, programming and controlling is user-friendly and 21 CFR Part 11 compliant. Control of pH/DO, medium temperature, shaking frequency and gas flow rate and mixture is possible.

Pumping system

Pumping system

The control unit incorporates three peristaltic pumps which allow aseptic fluid management. This enables simple regulation of the pH-value, feeding and the transfer of the culture media (scale-up or processing).

Gas mixing

Gas mixing (FlowCon)

The SB10-X control unit has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers (N2, O2, CO2 and air or gas mixture) ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor. Step limits can be freely programmed by the user. Integrated air filters and back pressure valves ensure interference free and long operating times for the FlowCon.


We subject all our shaking machines to an elaborate final test before delivery and send the test protocols to our customers.




Includes 6 module

- Shaker module ES-X
- Control unit


- Tray module
- Vessel module
- Cable and tube management module
- Filter heater

Shaken bioreactor

Working volume

4 L - 12 L

Total bag volume

approx. 24 L

(bioreactor incl. shaker

approx. 75 kg without liquid

Required footprint
(excl. control unit)

669 mm x 646 mm

Technical data subject to change

Dimensions (in mm) shaken bioreactor


Shaker module ES-X


modified ES-X benchtop shaker

Shaking diameter

50 mm (orbital motion)
shaking diameter is adjustable
(12.5 mm, 25 mm, 50 mm)

Shaking speed

20 rpm - 140 rpm
(when used with SB10-X)

Loading, maximum

25 kg

Setting, digital

1 rpm

Accuracy, absolute

± 0.5 rpm

Active brake


Ambient temperature

5°C - 40°C


stainless steel 1.4301

Mains connection


110 - 120 V / 50-60 Hz


95 - 105 V / 50-60 Hz

Temperature control


resistance heater (100 W),
integrated in tray module

Temperature max.

40 °C

Temperature min.

approx. ambient temperature (no cooling)

Temperature accuracy

± 0.25 °C (at 37 °C)

Temperature distribution

8 mm thick aluminum plate provides homogenous temperature distribution of resistance heater

pH and DO control

pH measurement

principle: optical chemosensor,
integrated in tray module


pH 5.5 – pH 8.5

Accuracy (chemosensor)

± pH 0.05 at pH 7 with one point calibration
± pH 0.10 at pH 7 with pre-calibration


< pH 0.005 per day

Temperature range

up to 50 °C

DO measurement

principle: optical chemosensor,
integrated in tray module


0 % – 100 % DO

Accuracy (chemosensor)

± 0.1 % O2 at 20.9 % O2

Accuracy (system)

± 10 % DO


< 0.015 % O2 per day

Temperature range

up to 50 °C

Filter heater


1 exhaust filter




resistance heater (6 W)

Max. temperature

approx. 40 °C at 23 °C ambient temperature


orange LED light indicates that filter heater is active and working

Control unit




approx. 30 kg

Required footprint

698 mm x 328 mm


Stainless steel 1.4301


2 x CAN-Bus
Optional: EMI-60, NET-60

Dimensions (in mm)

Massbild Steuermodul

Touch screen


12.1’’ screen (resistive)
front is splash proof and dust protected (IP 64)


1024 x 768


passive with no fan

Main memory

2 GB (1.6 GB usable)


4 x USB, 2 x RS 232,

2 x LAN, 1 x VGA



Kuhner Insight application software


pH & DO, shaking speed, temperature, gas flow and mixture, pressure


pre-calibrated sensors in bag


one point recalibration is possible


Win 7 & 10


21 CFR Part 11 compliant



3 peristaltic pumps


Watson-Marlow 313 VBM/D




70 W

Maximum Speed

100 rpm


1.6 mm wall thickness

Delivery Pressure

max. 2bar

Tube ID (mm)

Flow rates (mL/min) at 100 rpm















Gas mixing device (FlowCon)

Number of mass flow controllers


Ambient conditions

5 °C – 40 °C, max. 85 %rH


up to 4 gases (air, oxygen, nitrogen, carbon dioxide)
different gas mixtures
can also be connected
push in connector for tubes OD 6 mm
required input pressure: 1 bar – 2 bar


1 (tube fitting optimal for tubing 9 x 1 mm)
Flow rates:

N2: 0.1 L min-1 – 1.99 L min-1

O2: 0.1 L min-1 – 1.99 L min-1

C2: 0.1 L min-1 – 1.4 L min-1

air: 0.1 L min-1 – 1.99 L min-1

accuracy: ± 1.3 % of full scale

Pressure measurement

gas output: pressure sensor DELOS SI (Jumo),
integrated in FlowCon

Differential pressure

0 mbar – 100 mbar (programmable)

Gas filter

integrated, prevents blocking of mass flow controllers

Technical data subject to change

Overview options

3L-vessel module

With the 3L-vessel module, users can now perform cell cultivations in smaller working volumes ranging from 1.5 to 4.5 L. The 3L-vessel simply replaces the standard 10L-vessel on the SB10‑X shaking table. The smaller module is offered as an option in addition to the standard SB10‑X, or users can purchase the shaking bioreactor with just the 3L-vessel.

A retrofit kit is also available for current users of the SB10-X bioreactor who could benefit from the smaller module. The kit includes additional components for the tray module, a 3L-vessel module, and the software and firmware updates. The shaking diameter should be adjusted to 25 mm. Suitable 3L-single-use cultivation bags are ready for purchase.

We are happy to provide data on kLa, mixing time, volumetric power input, heating time, and evaporation rate to help our customers scale up their bioprocesses.

3L-vessel module


IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.


Overview Accessories

Single-use bag

Kuhner offers three different single-use bag types to meet all user needs. Each pack contains three bags.
These gamma-irradiated bags for the Kuhner shaken bioreactor require no additional mixing device, enable quick set up times and eliminate elaborate cleaning and sterilising procedures. All bags have a working volume from 4 litres up to 12 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling.

The standard bag (SMX760001) has built in optical sensors for pH and DO which allow non-invasive online measurement and control of pH and DO. This standard bag is suitable for ATF perfusion.
The basic single-use bag (SMX760002) is the same as the standard bag, but has no optical sensors.
The perfusion bag (SMX760003) has two exhaust filters and is equipped with additional tubes and ports for TFF perfusion. ATF perfusion is also possible.

Order number



3 x standard single-use bags for SB10-X with optical chemosensors for pH/DO and tubes


3 x basic single-use bags for SB10-X
with no optical sensors


3 x perfusion single-use bags for SB10-X with 2 exhaust filters, optical chemosensors for pH/DO and additional tubes

Light protection jacket

This light protection jacket is easily placed over the vessel module. It protects light sensitive media or products from UV and artificial light as well as daylight.

Order number



Light protection jacket


Overpressure valve & glass fibers (spare parts)

Order number



Red overpressure valve, OD 9mm


2 glass fibers for pH/DO probes, length 40cm


MagFix is a set of 8 magnetic tube holders to help secure and guide all the tubing. It consists of 6 units for tubing OD 6mm/11mm and 2 units for tubing OD 15mm. The tubing fits easily into the slots on each unit. These magnetised holders can be attached to various steel surfaces on the vessel module, cable management module and shaker module of the SB10-X. The magnets on these units are so strong that they are also effective through the light protection jacket.

Order number






Two additional interfaces, EMI-60 and NET-60, are available as options.


The EMI-60 has several interfaces: two analogue outputs and two individually configurable alarm relays.

NET-60 allows the machine to be connected to almost all conventional Bus-systems and to OPC servers.


Kuhner Notes


Characterisation of SB10-X single-use bioreactor

Distribution and mixing times of microcarriers

New Orbital Single Use Bioreactor

Orbital Mixing as an Alternative to Stirred-Tank Bioreactors

Characterization of orbitally shaken single-use bioreactors

Baculovirus-based r-protein production in orbitally SUB

Cultivation of entomopathogenic fungi in orbitally shaken bioreactors

Orbital Shaken Bioreactors
The Scale Up Fast Track

Flexible production of an Fc-fusion protein

In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.

SB10-X – Our orbital shaken bioreactor for single-use bags

The SB10-X is an orbital shaken benchtop bioreactor system for the cultivation of human, mammalian and plant cells in a single-use bag. It has a working volume from 4 litres up to 12 litres. Also perfusion processes can be easily realized (ATF / TFF).

The SB10-X can be used in research, process development or as a pilot scale bioreactor

SB10-X single-use bioreactor - Bag unpacking (part 1)

In this video, you can see how to unpack the single-use bag before installing it into the SB10-X.

SB10-X single-use bioreactor - Bag installation (part 2)

In this video, you can see how to install the single-use bag into the SB10-X.

SB10-X single-use bioreactor - 3L-vessel module

With the 3L-vessel module, users can now perform cell cultivations in smaller working volumes ranging from 1.5 to 4.5 L.

The following flyers provide information about the machines and function-specific solutions.

Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.

Stem cells
Cell culture
Plant cells & algae


Adolf Kühner AG (Headquarters)

Dinkelbergstrasse 1

CH-4127 Birsfelden (Basel)



We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently. 

GMP compliant production of shakers and bioreactors

For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

Read more


The world's most reliable shaking machines built in-house in Switzerland.

«Kuhner respects your privacy»

We use cookies to provide you with an innovative experience on our website and to improve our communication with you. Cookies on this website are used to ① improve the user experience (strictly necessary Cookies) ② personalize information about your country and ③ learn how our website is used.

close x

Necessary for the usability of the website (always active)

Necessary cookies help to make a website usable by enabling basic functions such as page navigation and access to secure areas of the website. The website cannot function properly without these cookies.

Save country and language settings for convenience 

Country and language cookies allow our website to remember information such as: Your preferred language or country of residence. You will always receive the right information and valid offers tailored to your country.

Statistics for performance measurement 

Statistics cookies help us as a website operator to understand how visitors interact with websites by collecting and reporting information anonymously. From the statistics, we gain insights in order to constantly adapt the further development of our communication to the needs of the visitors.

Further information on the handling of cookies and information on data protection can be found here. You can adjust your cookie preferences in the settings.