The SB10-X is an orbital shaken benchtop bioreactor
system for the cultivation of human, mammalian and plant cells in a single-use
bag. It consists of six modules (excluding the bag) and has a working volume
from 4 litres up to 12 litres.
The SB10-X can be used in research, process development or as a pilot scale bioreactor.
Cultivation of human, mammalian and plant cells
Fast, simple set up
Online measurement and control of pH and DO
Three different types of single-use bags available
5 year warranty
The cylindrical vessel accepts a single-use 3D bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The standard bag has built-in optical sensors for pH/DO allowing online measurement and control of pH and DO in the medium. Two other bag types are available: a basic single-use bag with no optical sensors and a perfusion bag for ATF and TFF perfusion.
The orbital motion of the SB10-X ensures efficient liquid mixing. It facilitates high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB10-X uses the unique Kuhner direct drive which is very robust and provides accurate control of the orbital speed.
The SB10-X fills a gap in the scale-up chain from commonly used
single-use flasks all the way up to 50 L and 200 L bioreactors. The consistent
hydrodynamics of shaken bioreactors provide reproducible cultivation conditions
throughout the volume range. The culture from the SB10-X can serve as a
preculture for the SB50-X and SB200-X. The SB10-X can also be used
as a pilot scale bioreactor.
The tray module, which is fixed onto the ES-X shaker module, contains integrated heating and the connections for online pH and DO measurement.
Special emphasis has been placed on developing a user-friendly vessel
module. The vessel together with its bag can be easily detached by the user and
carried to the clean bench.
SB10-X, there are two vessel modules available with different working volumes.
The standard module is a 10L-vessel module with a working volume of 4 to 12 L.
The 3L-vessel module has a working volume of 1.5 to 4.5 L and is either available
as an option in addition to the standard SB10-X or customers may purchase the
SB10-X with the 3L-vessel only.
A filter heater for one exhaust filter is placed above the vessel module. The magnetic mounting makes handling the filter heater very simple and allows its location in various places. The filter heater combats water vapour condensation in the outlet air preventing blockages in the membrane
The compact and functional design of the control unit provides easy
handling and monitoring of the bioprocess parameters. The control unit has an integrated
and adjustable 12” touch screen with USB and Ethernet connections. The Kuhner
Insight application software for data recording, calibration, programming and
controlling is user-friendly and 21 CFR Part 11 compliant. Control of pH/DO, medium temperature, shaking
frequency and gas flow rate and mixture is possible.
The control unit incorporates three peristaltic pumps which allow aseptic fluid management. This enables simple regulation of the pH-value, feeding and the transfer of the culture media (scale-up or processing).
The SB10-X control unit has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers (N2, O2, CO2 and air or gas mixture) ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor. Step limits can be freely programmed by the user. Integrated air filters and back pressure valves ensure interference free and long operating times for the FlowCon.
Includes 6 module
- Shaker module ES-X
- Tray module
4 L - 12 L
Total bag volume
approx. 24 L
approx. 75 kg without liquid
669 mm x 646 mm
Technical data subject to change
modified ES-X benchtop shaker
50 mm (orbital motion)
20 rpm - 140 rpm
± 0.5 rpm
5°C - 40°C
stainless steel 1.4301
220 - 240 V / 50-60 Hz
110 - 120 V / 50-60 Hz
95 - 105 V / 50-60 Hz
resistance heater (100 W),
approx. ambient temperature (no cooling)
± 0.25 °C (at 37 °C)
8 mm thick aluminum plate provides homogenous temperature distribution
of resistance heater
principle: optical chemosensor,
pH 5.5 – pH 8.5
± pH 0.05 at pH 7 with one point calibration
< pH 0.005 per day
up to 50 °C
principle: optical chemosensor,
0 % – 100 % DO
± 0.1 % O2 at 20.9 % O
± 10 % DO
< 0.015 % O2 per day
up to 50 °C
1 exhaust filter
resistance heater (6 W)
approx. 40 °C at 23 °C ambient temperature
orange LED light
indicates that filter heater is active and working
approx. 30 kg
698 mm x 328 mm
Stainless steel 1.4301
2 x CAN-Bus
12.1’’ screen (resistive)
1024 x 768
passive with no fan
2 GB (1.6 GB usable)
4 x USB, 2 x RS 232,
2 x LAN, 1 x VGA
Kuhner Insight application software
pH & DO, shaking
speed, temperature, gas flow and mixture, pressure
pre-calibrated sensors in bag
one point recalibration is possible
Win 7 & 10
21 CFR Part 11 compliant
3 peristaltic pumps
Watson-Marlow 313 VBM/D
1.6 mm wall thickness
Tube ID (mm)
Flow rates (mL/min) at 100 rpm
Number of mass flow controllers
5 °C – 40 °C, max. 85 %rH
up to 4 gases (air, oxygen, nitrogen, carbon dioxide)
1 (tube fitting
optimal for tubing 9 x 1 mm)
air: 0.1 L min-1 – 1.99 L min-1
gas output: pressure sensor DELOS SI (Jumo),
0 mbar – 100 mbar (programmable)
integrated, prevents blocking of mass flow controllers
Technical data subject to change
With the 3L-vessel module, users can now perform cell cultivations in smaller working volumes ranging from 1.5 to 4.5 L. The 3L-vessel simply replaces the standard 10L-vessel on the SB10‑X shaking table. The smaller module is offered as an option in addition to the standard SB10‑X, or users can purchase the shaking bioreactor with just the 3L-vessel.
A retrofit kit is also available for current users of the SB10-X bioreactor who could benefit from the smaller module. The kit includes additional components for the tray module, a 3L-vessel module, and the software and firmware updates. The shaking diameter should be adjusted to 25 mm. Suitable 3L-single-use cultivation bags are ready for purchase.
We are happy to provide data on kLa, mixing time, volumetric power input, heating time, and evaporation rate to help our customers scale up their bioprocesses.
IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.
Kuhner offers three different single-use bag types to
meet all user needs. Each pack contains three bags.
These gamma-irradiated bags for the Kuhner shaken bioreactor require no additional mixing device, enable quick set up times and eliminate elaborate cleaning and sterilising procedures. All bags have a working volume from 4 litres up to 12 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling.
The standard bag (SMX760001) has built in optical sensors for pH and DO which allow non-invasive online measurement and control of pH and DO. This standard bag is suitable for ATF perfusion.
The basic single-use bag (SMX760002) is the same as the standard bag, but has no optical sensors.
The perfusion bag (SMX760003) has two exhaust filters and is equipped with additional tubes and ports for TFF perfusion. ATF perfusion is also possible.
3 x standard single-use bags for SB10-X with optical chemosensors for pH/DO and tubes
3 x basic single-use bags for SB10-X
3 x perfusion single-use bags for SB10-X with 2 exhaust filters, optical chemosensors for pH/DO and additional tubes
This light protection jacket is easily placed over the vessel module. It protects light sensitive media or products from UV and artificial light as well as daylight.
Light protection jacket
Red overpressure valve, OD 9mm
2 glass fibers for pH/DO probes, length 40cm
MagFix is a set of 8 magnetic tube holders to help secure and guide all
the tubing. It consists of 6 units for tubing OD 6mm/11mm and 2 units for
tubing OD 15mm. The tubing fits easily into the slots on each unit. These
magnetised holders can be attached to various steel surfaces on the vessel
module, cable management module and shaker module of the SB10-X. The magnets on
these units are so strong that they are also effective through the light
Two additional interfaces, EMI-60 and NET-60, are available as options.
The EMI-60 has several interfaces: two analogue outputs and two individually configurable alarm relays.
NET-60 allows the machine to be connected to almost all conventional Bus-systems and to OPC servers.
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
Der SB10-X ist ein orbital geschüttelter Bioreaktor zur Kultivierung von humanen, tierischen und pflanzlichen Zellen in Einwegbeuteln. Das Bioreaktorsystem (SB10-X) besitzt ein Arbeitsvolumen von 4L bis 12L und besteht aus sechs Modulen (exkl. Einwegbeutel).
Der SB10-X kann in der Forschung, Prozessentwicklung oder als Pilotscale-Bioreaktor eingesetzt werden.
Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
The world's most reliable shaking machines built in-house in Switzerland.