Select a different language or country,
to view content for your location.
English
en
German
de
Worldwide
Apply
×

Feeding Technology for Fed-Batch

Feeding Technology for Fed-Batch
Overview
Overview
FeedPlates®
FeedBeads®
Science
Videos
FAQ
PDF

Are you searching for the most productive strain for a fed-batch process?

The Kuhner Feeding Technology provides an innovative tool for efficient high-throughput screenings in fed-batch mode. The diffusion-driven fed-batch system is delivered sterile and ready-to-use for the instant application in the lab. The Kuhner Shaker GmbH offers three items applying the Kuhner Feeding Technology: Feed Plate® , FeedBeads® and FeedTubes®.

By using the fed-batch mode instead of batch cultivations, overflow metabolism with undesired side-product formation can be significantly reduced and oxygen limited conditions can be avoided. Thereby, screening results are improved and a higher product formation is achieved. 

Performance

Superior outcome in screening

Scale-up

For efficient scale-up

Reproducible conditions

Reproducible results

Applikation

Application


Cultivation conditions decide about which strain is chosen as the best

Using the Kuhner Feeding Technology, cultivation conditions of the strain screenings are more comparable to those on a production scale, since overflow metabolism, pH inhibition, and oxygen limitation are prevented. Thus, an unfavorable strain selection is avoided already early on. Comparable and reproducible conditions in screening allow you to identify the optimal production strain already at an early time point. Consequently, this reduces costs as well as time-consuming process adaptations during later stages in process development.


The Benefits of Feeding Technology for Your Screening

Icon Schraubenschlüssel

Universal tool

for MTP, shake QWflasks and tubes

Icon Podest

Various substrates and release rates

for individual needs

Icon Schieberegler

Fast and easily integrated

sterile and ready-to-use


Reducing negative influences

O2-limitation, by-products and overflow, pH-shifts


Improving Screening

controlled cell metabolism and higher significance of results


Simplified scale-up

starting process development one step earlier


Selection of the best strain

for later production process


High product yields

improved cell metabolism
extended cultivation time


Reduction of costs

avoidance of unforeseen development costs


Better screening results


FeedPlates®

Glucose

Low, Standard and High release

Type Article Substrate Release after 48 h *
96 well, round SMFP00701 Glucose 3 mg
96 well, round SMFP01001 Glucose 5 mg
96 well, round SMFP04001 Glucose 9 mg
96 well, round SMFP08001 Glucose 10 mg
96 well, square SMFP00702 Glucose 4 mg
96 well, square SMFP01002 Glucose 6 mg
96 well, square SMFP04002 Glucose 12 mg
96 well, square SMFP08002 Glucose 14 mg
48 well, round SMFP08004 Glucose 23 mg
24 well, eckig SMFP00703 Glucose 18 mg
24 well, eckig SMFP01003 Glucose 30 mg
24 well, eckig SMFP04003 Glucose 48 mg
24 well, eckig SMFP08003 Glucose 60 mg

Glycerol

Low and High release

Type Article Substrate Release after 48 h *
96 well, round SMFP11001 Glycerol 3.5 mg
96 well, round SMFP12001 Glycerol 6.5 mg
96 well, square SMFP11002 Glycerol 5 mg
96 well, square SMFP12002 Glycerol 10 mg
48 well, round SMFP11004 Glycerol 10 mg
48 well, round SMFP12004 Glycerol 19 mg
24 well, square SMFP11003 Glycerol 27 mg
24 well, square SMFP12003 Glycerol 40 mg

Glucose

High release plus

Type Article Substrate Release after 48 h *
96 well, round SMFP02701 Glucose 15 mg
96 well, square SMFP02702 Glucose 18 mg
48 well, round SMFP02704 Glucose 36 mg
24 well, square SMFP02703 Glucose 100 mg

* Release determined at 37 ° C in 10 mM PBS buffer, pH 7.4

For your high-throughput screening, we recommend that you check the substrate release under your own cultivation conditions and with your own media.

For samples, orders or a personal consultation contact us!

FeedPlates®

Easy application of fed-batch in shake flasks

Packing size: 25 beads / pack

Glucose

Article Bead size Substrate Release after 48 h *
SMFB01001 Ø 12 mm, h= 3 mm Glucose 32 mg
SMFB02001 Ø 12 mm, h= 3 mm Glucose 40 mg
SMFB08001 Ø 12 mm, h= 3 mm Glucose 75 mg
SMFB08002** Ø 24 mm, h= 3 mm Glucose t.b.d.
**packaging size: 20 beads/pack

Special substrates

Article Bead size Substrate Release after 48 h *
SMFB78011 Ø 12 mm, h= 3 mm Glutamin t.b.d.

Glycerol

rticle Bead size Substrate Release after 48 h *
SMFB11001 Ø 12 mm, h= 3 mm Glycerol 28 mg
SMFB12001 Ø 12 mm, h= 3 mm Glycerol 40 mg

* Release determined at 37 ° C in 10 mM PBS buffer, pH 7.4

For your high-throughput screening, we recommend that you check the substrate release under your own cultivation conditions and with your own media.

For samples, orders or a personal consultation contact us!

Kuhner Notes


Poster

Kuhner FeedingTechnology

Fed-Batch Micro Plates

Fed-batch Mode in Shake Flasks

Simple shake flask cultivation of Komagataella phaffii with constant glycerol feed


Publications


In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.

Feeding Technology - Application of the Kuhner FeedPlate®

Learn how to perform a fed-batch in a microtiter plate.

Discover more about Feeding Technology on our website:  https://www.feedingtechnology.com

Good to know

What does the FeedPlate® do?

The FeedPlate® allows the simulation of a Fed-Batch cultivation on a very small scale. Using the FeedPlate®, you can easily obtain reliable statements how your strains behave under Fed Batch conditions in the screening process.

What is the FeedPlate®?

The FeedPlate® consists of a commercially available microtiter plate with our special polymer matrix at the bottom of each well immobilized. This matrix contains the substrate.

How does the FeedPlate® work?

The FeedPlate® is a polymer-based release system. After the microtiter plate comes into contact with culture medium, the release of the substrate starts according to an evendefined rate over a period of up to 7 days.

Do I need special media to work with the Feed Plate®?

No. The Feed Plate® is a physicochemical system and does not require enzymes or special media or additives. The release kinetics is mainly dependent on the temperature and osmolarity of the medium used. Therefore, we refer to the release rate in the product catalog to a reference medium or cultivation conditions.

How many samples can I process with the FeedPlate®?

The FeedPlate® is currently available as standard in 24-well, 48-well and 96-well format, which allows you to process many samples in parallel in a reliable manner. This allows you to screen easily and quickly in high throughput.

What is the format of the FeedPlate®?

The FeedPlate® is available in commercially available microtiter plate formats. You choose which format you want to use. We produce special formats on request.

However, I only use microtiter plates from a certain manufacturer.

No problem, we can theoretically dose our matrix into any type of microtiter plate (cell culture tubes, 6-, 12-, 24-, 48- or 96-well MTPs), also different materials (PP, PS, etc.) or plates from certain manufacturers are possible (on request).

Are substrates other than C sources possible?

In addition to the standard carbon source glucose, we also offer other substrates such as maltose and fructose, and complex media such as yeast extract or other substrate classes such as nitrogen and phophate are also possible. Please contact us!

I need a high OTR (oxygen transfer rate). Can I still work in the 96 Deepwell plate?

Yes, that is not a problem. As long as you cultivate in Fed-Batch mode, oxygen supply should not be a problem. If you have any questions about how to reach a specific OTR, we will be happy to advise you and provide appropriate literature.

How should the FeedPlate® be stored?

The FeedPlate® should be stored dry at room temperature. Direct sunlight should be avoided.

How stable is the FeedPlate®?

The substrates are usually incorporated into the matrix as stable crystalline substances. Shelf life is therefore a question of sterility. The Feed Plate® is sterilized by beta radiation and is at least durable for one year.

You have a question that we do not answer here?

Contact us: Call us or write us an e-mail. We will be happy to answer your open questions.

The following flyers provide information about the machines and function-specific solutions.

Contact

Adolf Kühner AG (Headquarters)

Dinkelbergstrasse 1

CH-4127 Birsfelden (Basel)

Switzerland

Services

We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently. 

GMP compliant production of shakers and bioreactors

For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

Read more

Bild

The world's most reliable shaking machines built in-house in Switzerland.

We use cookies in order to optimize our website for you. If you continue reading on this website, you agree to the use of cookies.
You can find more information on the page about Cookies.