Select a different language or country,
to view content for your location.
English
en
German
de
Worldwide
Apply
×

SB200-X single-use bioreactor

SB200-X single-use bioreactor
Overview
Overview
Technical Data
Options
Accessories
Science
FAQ
PDF

Orbital shaken bioreactor for single-use bags

The SB200-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 50 litres up to 200 litres for the SB200-X ensures easy scale-up from microtiter plates to shaking ­ asks and all the way up to 200 litres. This user friendly system can be easily deployed in research, process development or production.

The SB200-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).

Performance characteristics

  • Large capacity (200L working volume) single use bioreactor system
  • Cultivation of human, mammalian and plant cells
  • Fast, simple set up
  • Easy scale-up
  • Online measurement of pH and DO

5 year warranty

5 year warranty

Single-Use

Single-Use

Scale-Up

Scale-Up

GMP

GMP


Bag

Single-use bag for a fast and simple set up

The vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.

optische Sensoren pH/DO

Orbital motion

Orbital motion

The orbital motion of the SB200-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB200-X uses the unique Kuhner direct drive which provides acurate control of the orbital speed.


Scale-Up

Trouble free scale-up

Another advantage of the SB200-X is easy scale-up from microtiter plates all the way up to 200 litres. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range. 


Control unit

Control unit and online measurement

The bespoke control unit, designed by Kuhner, uses the latest touch-screen technology and Kuhner Insight software for data recording, calibration, programming and controlling. Technical controls for 21 CFR Part 11 compliance are built into the software.

The SB200-X can be heated or cooled. Temperature ramps are easily programmed and processed.
Duplex online measurement of each parameter (dissolved oxygen and pH value) is available ensuring greater process safety.

Online-Messung

Pumping system

Pumping system

Pumpensystem

The control unit incorporates two small and one big peristaltic pump. This enables simple regulation of the pH value, feeding and the transfer of the culture media (scale-up or processing).


Gas mixing

Gas mixing

The SB200-X has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor by reducing the gas flow in the first step (1. limit) and shutting off the gas flow in the second step (2. limit). Step limits can be freely programmed by the user. Integrated air filters and back pressure valves ensure interference free and long operating times for the FlowCon.

Gasmischung

Filter heater

Filter heater

Filterheizung

A height adjustable filter heater, with up to two exhaust filters, is installed above the vessel. Special emphasis has been placed on developing a user friendly unit. The filter heater combats any water vapour condensation in the outlet air which might cause the membranes to block. The temperature is monitored and controlled by Kuhner Insight software.

Shaker


SMX7100

Shaker speed

max. 80 rpm

Shaker diameter

50 mm (orbital motion)

Weight

approx. 400 kg without liquid

Accuracy, absolute

± 0.5 rpm

Setting, digital

1 rpm

Active brake

adjustable

Interface

CAN-Bus, RS232

Temperature

up to 40°C

Cooling

cooling coils are incorporated for connection to an external cooling system (pressure < 0.2bar)

Dimensions (in mm) shaker

Standarddatei

Mains connection

SMX1021

220 - 240 V / 50-60 Hz

SMX1023

110 - 120 V / 50-60 Hz

SMX1024

95 - 105 V / 50-60 Hz

Reader Box-X

pH measurement

principle: optical chemosensor

Range

pH 5.5 – pH 8.5

Accuracy (chemosensor)

± pH 0.05 at pH 7 with one point calibration
± pH 0.10 at pH 7 with pre-calibration

Drift

< pH 0.005 per day

Temperature range

up to 50°C

 

DO measurement

principle: optical chemosensor

Range

0% – 100% DO

Accuracy (chemosensor)

± 0.1% O2 at 20.9% O2

Accuracy (system)

± 10% DO

Drift

< 0.015% O2 per day

Temperature range

up to 50°C

Filter heater


SMX7120

Capacity

2 exhaust filters

Temperature range

Ambient temperature up to 45°C,
monitored and controlled by Kuhner Insight software

Control unit

Steuerwagen


SMX7110

Weight

120kg

Monitor

15'' touchscreen monitor

Dimensions (in mm) control unit

Massbild Steuermodul

Kuhner Insight Software

Software

for monitoring, calibration, controlling

Monitoring

O2, pH, shaking speed, temperature

Calibration

pre-calibrated sensors in bag

Recalibration

one point recalibration is possible

Applicable

XP/Vista/Win7&8

GMP

21 CFR Part 11 compliance (audit trail)

Pumps

2 small pumps (A+B)

Watson-Marlow 313VBM/D

Gear

10

Maximum Speed

100 rpm

Power

70 W

Rollers

3

Tube

1.6 mm wall tubing

Delivery Pressure

max. 2bar


1.6 mm wall tubing
Bore diameter (mm)

Flow rates (ml/min at 100 rpm)

0.5

3.4

0.8

7

1.6

27

3.2

100

4.8

220

6.4

360

8.0

500

1 big pump (C)

Watson-Marlow 520VBM/R2

Gear

10

Maximum Speed

400 rpm

Power

70 W

Rollers

2

Tube

2.4 mm wall lubing 

Delivery Pressure

max. 2bar


2.4 mm wall tubing
Bore diameter (mm)

Flow rates (ml/min at 100 rpm)

0.5

4.1

0.8

10.5

1.6

42

3.2

170

4.8

380

6.4

680

8.0

1040

9.6

1500

Gas mixing 

Number of mass flow controllers

up to 4

Ambient conditions

5-40 [°C], max. 85 [%rH]

Input

up to 4 gases, push in connector 6 [mm]
(air, oxygen,nitrogen, carbon dioxide)
(gas mixtures can also be connected)

Input pressure

1-2 bar

Output

1, (tube fitting for tube ID 13 [mm])

Flow rate

0 - 20 [sL/min]

Accuracy

±1.5% from the end value

Pressure sensor

integrated, 0-400 [mbar], programmable
prevents overpressure in the connected vessel

Air filter

integrated, prevents blocking the mass flow controllers

Back pressure valve

integrated, prevents reflux into the mass flow controllers

Controlled filter
heating box
for the outlet filters, prevents blocking of the filters caused by condensation
(only air inlet and outlet with silicon tubing)

C-Flex material

for the tubing, enables the user to connect additional components via tube welding

Technical data subject to change 

Overview options

Do you know this option?

Cultivate with a 50L single-use bag in an orbital shaken bioreactor SB200-X.


Cooling

The SB50-X has a cooling ring embedded in the base plate. Cooling is achieved by connecting an external cooling device. An integrated valve, controlled by the Software, regulates the set point temperature.


Tubing support bar

A tubing support bar is available for attaching the various tubes (feeding, medium, air and pH regulation) and keeping them tidy.

Schlauchdurchführungsbogen

Adapter 50L single-use bag

The stainless steel adapter enables to use a 50L single-use bag in the orbital shaken bioreactor SB200-X. The stainless steel adapter and 50L single-use bag can easily be installed and operated in the orbital shaken bioreactor SB200-X. This option gives the flexibility to cultivate with either a 50L or a 200L single-use bag in the same orbital shaken bioreactor.

50 L Adapter

IQ/OQ-Documentation

IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.

GMP

Overview accessories

Single-use bag for a fast and simple set up

The gamma irradiated bag for the Kuhner shaken bioreactor requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. The single-use bag has a working volume from 50 litres up to 200 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.

Order number

Description

SMX710002

Bag for SB200-X with optical sensors


Overpressure valve & glass fibers (spare parts)

Order number

Description

SMX870001

Red overpressure valve, OD 14mm

SMX821500

Glass fibers for pH/DO probes, length 1.5m


Interfaces

Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces. More.

Kuhner Insight Software

Poster

Fluid flow and mixing dynamics in a shaken bioreactor

Orbital Mixing as an Alternative to Stirred-Tank Bioreactors

Scale-up Production of Pharmaceutical Proteins in Plant Cell Suspensions

Orbital Shaken Bioreactor for Cell Cultivation

Orbital shaken bioreactors in the field of cell cultivation

Characterization of orbitally shaken single-use bioreactors

Orbital Shaken Bioreactors
The Scale Up Fast Track


Publications


In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.

Good to know

Is it possible to move the SB200-X with a manually operated fork lift?

Yes, no other equipment is required.

Is anything else required to fit the single-use bag?

No, the single-use bag is simply placed in the SB200-X vessel, positioned and then inflated.

Is the mechanical stress on the cells greater than with the stirred system?

No, shaken systems have a very homogenous and low local power input. This means that high local power inputs which are responsible for mechanical stress, simply don’t occur.

Is IQ-OQ documentation available for the shakers?

Yes

Can the machine be used in the GMP sector?

Yes

The following flyers provide information about the machines and function-specific solutions.

Contact

Adolf Kühner AG (Headquarters)

Dinkelbergstrasse 1

CH-4127 Birsfelden (Basel)

Switzerland

Services

We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently. 

GMP compliant production of shakers and bioreactors

For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

Read more

Bild

The world's most reliable shaking machines built in-house in Switzerland.

We use cookies in order to optimize our website for you. If you continue reading on this website, you agree to the use of cookies.
You can find more information on the page about Cookies.