The SB200-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 50 litres up to 200 litres for the SB200-X ensures easy scale-up from microtiter plates to shaking asks and all the way up to 200 litres. This user friendly system can be easily deployed in research, process development or production.
The SB200-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).
5 year warranty
The vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
The orbital motion of the SB200-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB200-X uses the unique Kuhner direct drive which provides acurate control of the orbital speed.
Another advantage of the SB200-X is easy scale-up from microtiter plates all the way up to 200 litres. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range.
The bespoke control unit, designed by Kuhner, uses the
latest touch-screen technology and Kuhner Insight software for data recording,
calibration, programming and controlling. Technical controls for 21 CFR Part 11
compliance are built into the software.
The SB200-X can be heated or cooled. Temperature ramps are easily programmed and processed.
Duplex online measurement of each parameter (dissolved oxygen and pH value) is available ensuring greater process safety.
The control unit incorporates two small and one big peristaltic pump. This enables simple regulation of the pH value, feeding and the transfer of the culture media (scale-up or processing).
The SB200-X has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor by reducing the gas flow in the first step (1. limit) and shutting off the gas flow in the second step (2. limit). Step limits can be freely programmed by the user. Integrated air filters and back pressure valves ensure interference free and long operating times for the FlowCon.
A height adjustable filter heater, with up to two exhaust filters, is installed above the vessel. Special emphasis has been placed on developing a user friendly unit. The filter heater combats any water vapour condensation in the outlet air which might cause the membranes to block. The temperature is monitored and controlled by Kuhner Insight software.
max. 80 rpm
50 mm (orbital motion)
approx. 400 kg without liquid
± 0.5 rpm
up to 40°C
cooling coils are incorporated for connection to an external cooling system (pressure < 0.2bar)
220 - 240 V / 50-60 Hz
110 - 120 V / 50-60 Hz
95 - 105 V / 50-60 Hz
principle: optical chemosensor
pH 5.5 – pH 8.5
± pH 0.05 at pH 7 with one point calibration|
± pH 0.10 at pH 7 with pre-calibration
< pH 0.005 per day
up to 50°C
principle: optical chemosensor
0% – 100% DO
± 0.1% O2 at 20.9% O2
± 10% DO
< 0.015% O2 per day
up to 50°C
2 exhaust filters
Ambient temperature up to 45°C,
15'' touchscreen monitor
for monitoring, calibration, controlling
O2, pH, shaking speed, temperature
sensors in bag
one point recalibration is possible
21 CFR Part 11 compliance
2 small pumps (A+B)
1.6 mm wall tubing
| 1.6 mm wall tubing|
Bore diameter (mm)
Flow rates (ml/min at 100 rpm)
1 big pump (C)
2.4 mm wall lubing
2.4 mm wall tubing
(ml/min at 100 rpm)
Number of mass flow controllers
up to 4
5-40 [°C], max. 85 [%rH]
up to 4 gases, push in connector 6 [mm]|
(air, oxygen,nitrogen, carbon dioxide)
(gas mixtures can also be connected)
1, (tube fitting for tube ID 13 [mm])
0 - 20 [sL/min]
±1.5% from the end value
integrated, 0-400 [mbar], programmable|
prevents overpressure in the connected vessel
integrated, prevents blocking the mass flow controllers
Back pressure valve
integrated, prevents reflux into the mass flow controllers
for the outlet filters, prevents blocking of the filters caused by condensation|
(only air inlet and outlet with silicon tubing)
for the tubing, enables the user to connect additional components via tube welding
Technical data subject to change
The SB50-X has a cooling ring embedded in the base plate. Cooling is achieved by connecting an external cooling device. An integrated valve, controlled by the Software, regulates the set point temperature.
A tubing support bar is available for attaching the various tubes (feeding, medium, air and pH regulation) and keeping them tidy.
The stainless steel adapter enables to use a 50L single-use bag in the orbital shaken bioreactor SB200-X. The stainless steel adapter and 50L single-use bag can easily be installed and operated in the orbital shaken bioreactor SB200-X. This option gives the flexibility to cultivate with either a 50L or a 200L single-use bag in the same orbital shaken bioreactor.
IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.
The gamma irradiated bag for the Kuhner shaken bioreactor requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. The single-use bag has a working volume from 50 litres up to 200 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
Bag for SB200-X with optical sensors
overpressure valve, OD 14mm
fibers for pH/DO probes, length 1.5m
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
Yes, no other equipment is required.
No, the single-use bag is simply placed in the SB200-X vessel, positioned and then inflated.
No, shaken systems have a very homogenous and low local power input. This means that high local power inputs which are responsible for mechanical stress, simply don’t occur.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
The world's most reliable shaking machines built in-house in Switzerland.