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ISF1-X incubator shaker

ISF1-X incubator shaker
Overview
Overview
Technical Data
Options
Accessories
Science
Videos
FAQ
PDF

Stackable incubator shaker for microbial, mammalian and plant cell applications

The ISF1-X incubator shaker is suitable for applications in modern laboratories, research centres and production facilities. It offers a large shaking capacity with a small footprint and also many features allowing straightforward, safe handling of product.

Performance characteristics

  • Up to three machines can be stacked
  • Large shaking capacity with small footprint

  • Adjustable shaking diameters

  • Heating and cooling

  • CO2 and controlled humidity options available

5 year warranty

5 year warranty

Cooling

Cooling

CO2 control

COcontrol

Humidity control

Humidity control


Quality

Reliability pays

All ISF1-X components are designed for durability and reliability. A Kuhner shaker is a cost effective investment in quality backed with a five year warranty.

Icon Warranty

Modularity

A system that grows with your needs

Up to three ISF1-X shaking incubators can be stacked together for greater shaking capacity. Stacking is a simple procedure without the need for special tools or stacking kits. Each machine operates independently and can be equipped with cooling if required. For cell cultivation the ISF1-X is available with CO2. If using microtiter plates or particularly long cultivation times in shake flasks, the controlled humidity option will help reduce evaporation.

ISF1-X Übersicht

Direct drive

The Kuhner direct drive cannot be matched

Direktantrieb

The maintenance-free direct drive is both long lasting and economical. The drive is protected from spills and the all stainless steel interior has a drain at the back for quick removal of any unwanted liquids. The incubator chamber can be cleaned with chemicals or sterilized using optional UV lighting. Kuhner’s direct drive system allows the shaking diameter to be changed at any time as well as guaranteeing low energy consumption.


Safe system

A safe system

An upward opening door fitted with a safety switch, means the ISF1-X is easy to load and unload. Automatic supervision of the mains voltage protects the shaker and in the event of a voltage spike the unit disconnects from the mains. The machine resumes operation at the set values once power is restored.


Innovation

Innovation Partners

The ISF1-X sets today’s standard for incubator shakers thanks to its technology and functionality. To keep it that way, Kuhner maintains close contact with well-known universities and experts. We constantly investigate new developments looking for opportunities to further optimise the design and performance of our machines.

Technical Data


SMX1501

SMX1503


SMX1501C*

SMX1503C*

Cooling

yes

yes

Humidity control

no

yes

Temperature minimum

ambient


- 15°C


(- 10°C)*

ambient


- 15°C


(- 10°C)*

Temperature maximum 

80°C (60°C)*

80°C (60°C)*

Humidity maximum

-

85 % r.h.

Power consumption

< 1500W

< 2000W

 * incubator shaker optimised for cell cultivation

Machine

Gas volume

395 l

Weight (with cooling)

210 kg

Illumination

LED

Operation menu in

German, French, English, Italian, Spanish

Interface, standard

CAN-Bus

Interface, optional

USB, Ethernet, digital, analogue

Ambient temperature

10°C up to 35°C

Dimensions (in mm)

Left sidewall

Cable feedthrough: PG 16 (ID 20mm)

Optional: 2nd cable feedthrough 

Temperature

Setting, digital

0.1°C

Accuracy, absolute

± 0.30°C (37°C)

± 0.25°C (37°C)*

Principle of sensor

Pt-100

Power of heating

1000W

Power of cooling (electric)

125…270W

Air circulation

300m3/h

Humidity


(SMX1503)

max. at 25..55°C

85% r.h.

Setting, digital

1% r.h.

Accuracy, absolute

± 2% r.h.

Principle of sensor

capacitive

Water refill

automatic

Water heater

300W

Door heater

100W

CO₂


(SMX1034)

Principle of sensor

Infrared, NDIR

Measuring range

0...20% CO2

Setting, digital

0.1%

Accuracy, absolute
(including non-linearity, calibration uncertainty, repeatability)

± 0.40% at 5% CO2


Temperature range

5...60°C

CO2-supply

max. 2 bar overpressure

Mains connection

SMX1021:

220 - 240 V / 50-60 Hz

SMX1022:

190 - 210 V / 50-60 Hz

SMX1023:

110 - 120 V / 50-60 Hz

SMX1024:

95 - 105 V / 50-60 Hz

Shaking unit  

Tray, size

F (800x420mm)

Loading, maximum

25kg

Setting, digital

1rpm

Accuracy, absolute 

± 0.5 rpm

Timer

1s ... 999h

Acceleration

controlled

Active brake

adjustable 

Stop on position

adjustable

Shaking motion  


Speed

* orbital, Ø 12.5mm

20...500 rpm

* orbital, Ø 25.0mm

20...400 rpm

* orbital, Ø 50.0mm

20...300 rpm

* linear, ↔ 12.5mm

20...400 rpm

* linear, ↔ 25.0mm

20...300 rpm

* linear, ↔ 50.0 mm

20…200 rpm

* can be changed

Technical data subject to change

Overview Options

Do know these options? 

Touchscreen with Kuhner Insight Software for simple operation, monitoring and programming of incubator shakers.

Icon Touchscreen

Have you heard about our new EcoDew®-Technology? Find more information under kuhner technology.

Icon EcoDew

New: Automatic door option
for ISF1-X

Icon automatische Tür

EPFL-table for 50ml bioreactors

EPFL-Tisch

CO₂ control

CO2 control is essential when working with mammalian cell cultures. Kuhner was the first company to manufacture and supply incubator shakers with CO2 control more than 15 years ago and continues to offer this option. More.


Cooling

All Kuhner incubator shakers can be fitted with cooling. The refrigeration system is regulated by a fully digital PID control ensuring minimal energy consumption. The cooling is switched on only when required as determined by the chamber temperature and set point. More.


Humidity control

Humidity control is an important factor in successful fermentation. Evaporation from microtiter plates, or when cultivating in flasks for long periods (e.g. cell cultures), can be significantly reduced with humidification.

To reduce evaporation from shake flasks or microtiter plates a water bath is placed inside the incubator. This water bath is fitted with an automatic water supply.

Our newly developed EcoDew®-Technology provides precise humidity control. More.


Illumination unit for photosynthesis (LED)

The ceiling of any Kuhner incubator shaker can be fitted with LED modules for the cultivation of phototrophic organisms. The control module allows full programming of night/day cycles and variable light intensity.

LED

UV lamp

As in a biological safety cabinet, the chamber of a Kuhner incubator shaker can also be sterilized with an integrated UV lamp. The lamp is situated to maximise irradiation of the chamber and shaking table. The UV lamp has a clearly marked external switch and when the door is opened, the UV-lighting is automatically switched off.

UV-Lampe

Light shade plate

Kuhner has designed a new light shade plate for the ISF1-X. This plate is easily placed in front of the door window to prevent radiation affecting the contents of the incubator chamber. It protects light sensitive media from UV, artificial and/or daylight.

Simply lift up the light shade plate to look at the cultures without opening the incubator door. When light protection is no longer required, the plate is easily removed.

Check out the Kuhner EquipNote "Light protection for Kuhner incubators".

Lichtschutzblende

Black window

Available for light sensitive medium or organisms. Any Kuhner incubator shaker can be delivered with blackened windows to prevent unwanted daylight or UV radiation inside the incubator.

Check out the Kuhner EquipNote "Light protection for Kuhner incubators".

Fenster schwarz

Automatic door

The ISF1-X incubator shaker with automatic door is designed for easy handling and maximum comfort. The automatic door is activated by pressing a button on the incubator shaker. A foot switch option is also available.

Automatische Tür

Foot switch labels

Labelling plates are now available for the ISF1-X foot switch that is used with the automatic door option. For a  stack of 2x or 3x ISF1-X incubator shakers with the automatic door feature, these labelling plates will allow the user to easily identify which foot pedal corresponds to each machine. 

Fusstaster Beschriftung

Touchscreen

This option includes a 9-inch touchscreen with our Kuhner Insight Software. The touchscreen has been designed for simple operation, monitoring and programming. It can also be retrofitted to existing incubator shakers. More.

Touchscreen

Pull-out table

The pull-out table is designed for the ISF1-X and ISF4-X incubator shakers as well as the Rack System. This remarkable device makes loading and unloading trays much easier.

Ausziehtisch

Dual table

The dual table is an easy and economical way of doubling the shaking capacity. The dual shaking table consists of two levels. Each level will accept an F size tray. However, with the dual table the shaking speed is limited to a maximum of 200 rpm.

Doppelschütteltisch

EPFL-table

Are you screening using large numbers of 50ml bioreactors (TPP, Falcon tubes etc.)? Then our new EPFL-table is the ideal solution. This table accepts up to five tube holders, each with a capacity of 24 x 50ml tubes. Each tube holder has its own clamp handle allowing it to be easily taken out of the incubator shaker and put on a clean bench while the other tubes keep on shaking. It’s also possible to shake microtiter plates on the EPFL-table, using Duetz 4-place coverclamps. If you have a Kuhner F-size tray (800 x 420mm), you can also clamp that on the table instead of individual holders. The TabCom Option is not possible with an EPFL-table. The TabCom Option is not possible with an EPFL-table. 

An EPFL-table with 4 clamp positions is also available for the ES-X Lab-Shaker.

EPFL

EPFL-table

EPFL

EPFL-table with different holders 

EPFL

F-size tray clamped on the EPFL-table

EPFL

Clamp handle 


Stainless steel

The outside surface of the ISF1-X is completely made of stainless steel. The design of the ISF1-X incubator shaker is optimized for the work in cleanrooms and GMP environments (21 CFR Part 11). Interface and software also comply with GMP regulations (GAMP5 Category 4).

Edelstahl

Kuhner TOM

Kuhner TOM (Transfer-Rate Online Measurement) measures online oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR) and the respiratory quotient (RQ) in up to 16 individual shake flasks for better process understanding and informed process development. TOM is easily installed in any Kuhner shaker and can be operated with various flask types and sizes including single use shake flasks. Kuhner TOM has zero footprint on the shaker tray as it is mounted above (see picture below). More.

Kuhner TOM

Perspex cover

To prevent accidental operation of the controllers, the panel can be protected by a perspex cover.

Perspex cover

TabCom

The TabCom option from Kuhner consists of a cable for power and data with the connection port integrated in the shaking table (see pictures below). A cable guide prevents the cable breaking and ensures secure data recording. The flexibility of TabCom means online measurement systems can be easily integrated.

Port_TabCom
TabCom_pull-out-table

IQ/OQ-Documentation 

IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.

GMP

Overview Accessories

Do you know this accessorie?

Need more space in your laboratory? Check out our stacking adapter or universal stacking adapter.


Universal tray

The universal tray can be fitted with clamps for Erlenmeyer flasks, test tube holders, microtiter plates, or custom made holders to suit your requirements. Rearranging these clamps and holders is quick and simple.

Order number

Description

SM3003

Universal tray FU (800 x 420 mm)

SM312500U

Clamp U-2500 for Ultra Yield flask from Thomson

Universaltablar
ISF1-X_FU-20tray_8Ultra-20Yield

FU-tray with 8x Ultra Yield flasks (2500 mL) from Thomson


Special universal tray

A special F-size universal tray (800x420mm) is now available to shake large Erlenmeyer flasks. It is ideal for cell culture.

This tray allows parallel shaking of 7 x 3L Corning Fernbach culture flasks or 7 x 5L Thomson Optimum Growth flasks at a maximum orbital diameter of 50mm. The FUM-V has no handles and a V support underneath the tray raises it above the clamping mechanism creating more space for flasks.

Clamps must be ordered separately. (Order number: SM313000F)

Order number

Description

SMX310001

Special universal tray, FUM-V with V support
(Clamps not included)

SM313000F

U-3000F clamp for Fernbach flasks:
1 x 5L Thomson flask or
1 x 3L Corning Fernbach culture flask

FUV_tray_cellculture

Tray with fixed clamps

The clamps are riveted onto this tray and cannot be removed. It offers maximum stability and will often hold a larger number of flasks than an universal tray.  

Ordner number

Description

Number of clamps

SM330025

F-25 ml

153

SM330050

F-50 ml

100

SM330100

F-100 ml

74

SM330125

F-125 ml

60

SM330150

F-150 ml

60

SM330200

F-200 ml

40

SM330250

F-250 ml

40

SM330300

F-300 ml

30

SM330500

F-500 ml

26

SM331000

F-1000 ml

16

SM331500

F-1500 ml

12

SM332000

F-2000 ml

9

SM332500U

F-2500U

8

SM333000

F-3000 ml

8

SM334000

F-4000 ml

5

SM335000

F-5000 ml

4

F_Tablar_mit_genieteten_Klammern_Kuhner

Tray for microtiter plates

Kuhner has designed trays specifically for microtiter plates. Handles at the front of the tray open and close the special clamping mechanism allowing plates to be loaded and unloaded. Trays of different heights (22, 47 and 77mm) allow the stacking of up to four microtiter plates. The trays are available in three sizes and suitable for all types of plates (deep well, low well, 24, 48 and 96 well).

Order number

Description

Number of microtiter plates

SM3503.22

F-MT.22

24 - 48

SM3503.47

F-MT.47

24 - 96

SM3503.77

F-MT.77

24 - 144

Tray_Microtiterplates

Tray for microtiter plates (springs)

Kuhner has designed a new tray for microtiter plates. This tray is fitted with springs which allows each well plate to be loaded and unloaded individually. The tray is available in three sizes: E (420x420mm), EX (500x420mm) and F (800x420mm).

 

This new tray is particularly suitable for the special holder with filter lid for deep well microtiter plates.

Order number

Description

Number of microtiter plates

SM3503A

F-tray (800x420mm) for deepwell or microtiter plates

1-24

F-Tablar für Mikrotiterplatten (Federn)
Tablare für Mikrotiterplatten (Federn)

F-tray (800 x 420 mm)


Tray with pins

Kuhner has designed a new F-size tray (800 x 420mm) which is ideal for cell culture. The F-V5000/7 tray allows parallel shaking of 7 x 3L Corning Fernbach culture flasks, 7 x 5L Corning Erlenmeyer flasks or 7 x 5L Thomson Optimum Growth flasks. Flasks are held in position by pins and are easily placed on the tray and removed just as quickly.

This tray has been designed for easy cleaning and efficient handling of samples.

Order number

Description

 SM335000S

F-V5000/7
F-tray with pins for 7 flasks:
- 3L or 5L Corning
- 5L Thomson

F-V5000_7

Tray with rubber mat

The whole tray is covered with a rubber mat and is particularly suitable for  shaking containers at low speed (e.g. blood bags).

Order number

Description

SM3603

Rubber mat FG (800 x 420 mm)

Tablar mit Gummimatte

Tray with sticky strips

A tray fitted with sticky strips is especially useful for flasks shaken at low speeds (up to 200rpm). The flask is placed directly on the sticky strip without the need for any other support. Flasks of all sizes can be used. Sticky strips are available individually or in sets and can also be supplied already fitted to a tray. The E-size tray accepts four strips, while the EX-size tray requires five strips and the F-size tray eight strips.

Ordner number

Description

SMX350001K

F-size tray with 8 sticky strips (clear)

SMX837001K

1 sticky strip: 385 x 85 x 3 mm (clear)

Tablar mit Klebestreifen

Tray with PC-plate for sticky strips

The PC-plate increases the adhesion of the sticky strips. This is particularly helpful if using flasks >500ml.

Order number

Description

SMX350007K

F-size tray with 8 sticky strips (clear)

SMX350005K

F-size tray with 2 big sticky strips (clear)

SMX837001K

1 sticky strip: 385 x 85 x 3mm (clear)

SMX837002K

1 big sticky strip: 395 x 385 x 3mm (clear)


Tablar mit PC-Platte für Klebestreifen

Dual tray

The dual tray offers two shaking levels and doubles the capacity. It is available in EXU, EX, FU and F size and the upper tray can be removed.
Please note the shaking speed is limited.

Order number

Description

SMX3015

two FU trays with 180mm distance (with holes)

SMX3016

two F trays with 180mm distance
(no holes for sticky strips / rubber mats)

SMX3017

two FU trays with 200mm distance (with holes)

SMX3018

two F trays with 200mm distance
(no holes for sticky strips / rubber mats)

Doppeltablar

Tray with support bars

This tray offers an alternative to both clamps and sticky mats and is ideal for tall containers. The tray is covered with a rubber mat and has an upright at each corner. The F-tray is fitted with six cross supports. These support bars securely clamp containers.

Order number

Description

Number of longitudinal girders

SM4130.6

FA-tray with rubber mat and 6 cross supports

2

F-Tablar mit Aufbausystem und Querträger

Tray for separating vessels

Kuhner has also designed a special tray for shaking separating vessels. This very flexible tray can take vessels of various sizes. It is also possible to add a second layer and double the capacity. Movable retaining rods allow vessels to be installed easily.

Order number

Description

Number of separating vessels

SM430250

FS-250

10

SM4302502

FS-250/2

20 (2 layers)

SM430500

FS-500

8

SM4305002

FS-500/2

16 (2 layers)

SM431000

FS-1000

6

SM4310002

FS-1000/2

12 (2 layers)

Tablare für Scheidetrichter

Tray for centrifuge tubes

The use of centrifuge tubes (e.g. 50ml Falcon tubes, TPP 50ml and 600ml tubes) in cell culture screening is well established. Kuhner offers special trays in two different sizes for this purpose. The EX tray has a capacity of 72 (3x24) 50ml centrifuge tubes while the larger F size tray can take 120 (5 x 24) 50ml tubes. A holder for TPP 600ml tubes is also available.

Order number

Description

Number of centrifuge tubes

SM3805

50ml Tube F-tray (EPFL)

5 x 24 = 120

Tablare für Zentrifugenröhrchen

2D-Bag-tray (Cryo)

This tray was developed to shake (orbital or linear) 2D-Bags. Rubber bands secure for example smaller blood and cryo bags. The tray is compatible with ISF1-X, ISF4-X, Rack System (SBM/SS-X), LS-X and ES-X shakers. It accepts various brands and sizes of pillow shaped single-use bags:

  • 1 x 20L (10L working volume) cultivation bags
  • 2 x 10L (5L working volume) cultivation bags
  • 10 x 500ml or 12 x 250ml cryo bag
Order number Description

SMX350301

F-tray for 2D-bag with working volume 1/5/10L

2D-Bag-Tablar (Kryo)

2D-Bag-tray (Flex)

This tray was developed for shaking (orbital or linear) different types of 2D-bag. The tray consists of an aluminium base with a flexible mounting system on top.The mounting system can be manually adjusted to facilitate the controlled fixing of different sized cultivation bags. The mounting bars on both longitudinal sides can be moved up and down to hold the cultivation bag in place, while rubber bands will secure the smaller blood and cryo bags. The F-2D-Bag-tray is compatible with ISF1-X, ISF4-X, Rack System (SBM/SS-X), LS-X and ES-X shakers. The tray accepts various brands and sizes of pillow shaped single-use bags:

  • 1 x 20L (10L working volume) cultivation bags
  • 2 x 10L (5L working volume) cultivation bags
  • 10 x 500ml or 12 x 250ml cryo bags

Visit our poster gallery and find more information on the poster "Characterisation of a RSB 20 L.pdf"

Order number Description

SMX350302

F-Tray for 2D-bag with working volume 1/5/10L

2D-Bag-Tablar (Kryo)

Floor stand

For a comfortable working height Kuhner offers floor stands for both the ISF1-X and LT-X incubator shakers. These are available in a choice of 400mm or 765mm high. 

Order number

Description

SM1560

400 mm high for 2 x ISF1-X

SM1561

765 mm high for 1 x ISF1-X

Untergestell

Floor stands with wheels

Floor stands with wheels are available for both the ISF1-X and LT-X incubator shakers. These stands enable the user to move the machines easily. However, the shaking speed is limited.

Order number

Description

SMX1560A

400 mm high for 2 x ISF1-X

SMX1561A

765 mm high for 1 x ISF1-X

SMX1040

Upgrade costs for castors (Polyamid)

*only for new machines

Untergestell mit Rollen

Universal stacking adapter

The universal stacking adapter allows other equipment to be placed on top of an ISF1-X incubator shaker, providing an additional work surface. Create more space in your laboratory with the universal stacking adapter.

Ordner number

Description

SMX1576

Universal stacking adapter on ISF1-X

Universal Stapeladapter

Stacking adapter

This stacking adapter is designed for stacking a LT-X on an ISF1-X incubator shaker. Extra work space on the adapter allows room for flasks or a small machine, as well as the LT-X, thus creating a greater working area.

Order number

Desciption

SMX1575

Stacking adapter (LT-X on ISF1-X)

Stapeladapter

Kuhner Insight Software

The user-friendly Kuhner Insight software was developed for supervision, monitoring, and recording of data. The software is easy to operate. More.

Kuhner Insight Software

Interfaces

Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces. More.

Kuhner Insight Software

Shelf

The ISF1-X incubator shakers can be fitted with a shelf allowing cultivation in petri dishes. One or two shelves can be placed above the shaking table and fixed to the chamber walls with four screws.

Order number

Description

SMX1572

Shelf for ISF1-X


Water bath

To reduce evaporation from shake flasks or microtiter plates a Teflon coated water bath can be placed inside the incubator. This water bath is not fitted with an automatic water supply and must be topped up manually

Order number

Description

SMX1533B

Humidity bath, coated, for ISF1-X and ISF4-X

Feuchtewanne

Light shade plate

Kuhner has designed a new light shade plate for the ISF1-X. This plate is easily placed in front of the door window to prevent radiation affecting the contents of the incubator chamber. It protects light sensitive media from UV, artificial and/or daylight.

Simply lift up the light shade plate to look at the cultures without opening the incubator door. When light protection is no longer required, the plate is easily removed.

Check out the Kuhner EquipNote "Light protection for Kuhner incubators".

Order number

Description

SMX1554

Light shade plate for ISF1-X/Z

Lichtschutzblende

Kuhner Notes

Light protection for Kuhner incubators:


Poster

Characterisation of a RSB 20 L

Evaporation and Aeration in Shaken Bioreactors

Orbital Shaken Bioreactors
The Scale Up Fast Track

Characterisation of a RSB 20 L

Characterization of Algal Cell Culture in Photobioreactors


In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.

ISF1-X - Automatic Door

The ISF1-X incubator shaker with automatic door is designed for easy handling and maximum comfort. The automatic door is activated by pressing a button on the incubator shaker. A foot switch option is also available.

ISF1-X - 3mm shaking diameter

Check out our ISF1-X shaker running with 1000 rpm (3mm shaking diameter).

Good to know

Do you need a special kit for stacking incubator shakers?

No, Kuhner machines do not need kits or special tools for stacking. Full instructions are provided in the User Manual.

Is linear shaking available?

Yes

Is it possible to stack ISF1-X and ISF1-W incubator shakers together?

Yes, but please make sure you stack the W-series shakers on top of the X-series and not vice versa. 

Can the machine be used in the GMP sector?  

Yes

Is IQ-OQ documentation available for the shakers?

Yes

The following flyers provide information about the machines and function-specific solutions.

Contact

Adolf Kühner AG (Headquarters)

Dinkelbergstrasse 1

CH-4127 Birsfelden (Basel)

Switzerland

Services

We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently. 

GMP compliant production of shakers and bioreactors

For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

Read more

Bild

The world's most reliable shaking machines built in-house in Switzerland.

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