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The Kuhner LT-X (Lab-Therm) incubator shaker
has the same capabilities as the larger ISF1-Z and ISF4-X models. The incubator
shaker was specially developed to address the shaking needs of the
biotechnology and pharmaceutical industries. The practical size of the LT-X
means it will fit in any laboratory.
Fits into any laboratory
Used in biotech and pharmaceutical industries
Accepts flasks up to 6 litres
Two units can be stacked without the need for special kits or tools
Humidity, CO2 and O2 control available
5 year warranty
CO2 control
O2 control
Humidity control
Shaking diameter
Dr. Shakers Helpful Hint N°4 explains what the shaking diameter
is and how you can determine the shaking diameter of your lab shaker.
Find information on the importance of the shaking diameter for biological cultivations in our Shaking Technology Forum: Why is the shaking diameter important?
Exceptional chamber height means flasks up to 6 litres capacity can be accommodated. If using smaller flasks, there is sufficient head height to fit one or two stationary trays above the shaking table. It is also possible to stack two LT-X together without the need for special kits or tools.
The maintenance-free direct drive guarantees long and economical performance from the LT-X. The shaking diameter can be changed at any time thanks to Kuhner’s unique direct drive system. The integrated drive is protected from spills and the all stainless steel interior has a drain at the back for any unwanted liquids. The chamber can be cleaned using chemicals or sterilized with optional UV lighting.
A door switch ensures the motor stops as soon as the door is opened. After closing the door properly, the motor restarts. The safety, reliability and quality of our shakers are of personal concern to everyone at Kuhner which is why all our machines are supplied with a 5 year warranty.
We subject all our shaking machines to an elaborate final test before delivery and send the test protocols to our customers.
The LT-X can be equipped with options for CO2, O2,controlled humidity, LED photosynthesis, UV lighting and much more. It is now also possible to integrate measuring data from Kuhner shakers with your company’s own network using Kuhner Insight software together with the NET-60 interface.
LT-X EcoDew® | LT-X Compressor |
108672 + 102011 | 108672 + 102008 |
LT-XC Basic | LT-XC EcoDew® | LT-XC Compressor |
108671 | 108671 + 102011 | 108671 + 102008 |
LT-X(C) Basic | LT-X(C) EcoDew® | LT-X(C) Compressor |
not available | included | 102010 |
LT-X(C) Basic | LT-X(C) EcoDew® | LT-X(C) Compressor |
102012 | 102012 | 102012 |
LT-X(C) Basic | LT-X(C) EcoDew® | LT-X(C) Compressor | |
Cooling | no | no | yes |
Humidity control | no | yes | no / yes |
Temperatur minimal | ambient + 10 °C | ambient
+ 10 °C | ambient – 15 °C (– 10 °C)* |
Temperatur maximal | 80°C (60°C)* | 80°C (60°C)* | 80°C (60°C)* |
Humidity maximal | - | 85 % r.h. | - / 85 % r.h. |
Power consumption | < 700W | < 1050W | < 880W / < 1150W |
* Shaking incubator optimized for cell cultures Technical changes reserved | |||
Gas volume | 260 l |
Weight (with cooling) | 170 kg |
Illumination | LED |
Operating menu in | German, French, English, Italian, Spanish |
Interface, standard | CAN-Bus |
Interface, optional | USB, Ethernet, digital, analogue |
Ambient temperature | 10°C up to 35°C |
Left sidewall
Cable feedthrough: PG 16 (ID 20mm)
Optional: 2nd cable feedthrough
Setting, digital | 0.1°C |
Accuracy, absolute |
± 0.30°C (37°C) ± 0.25°C (37°C)* |
Principle of sensor | Pt-100 |
Power of heating | 500 W |
Power of cooling | 90...155 W |
Air circulation | 160 m3/h |
max. at 25..55°C | 85% r.h. |
Setting, digital | 1% r.h. |
Accuracy, absolute | ± 2% r.h. |
Principle of sensor | capacitive |
Water refill | automatic |
Water heater | 180 W |
Door heater | 90 W |
Principle of sensor | Infrared, NDIR |
Measuring range | 0...20% CO2 |
Setting, digital | 0.1% |
|
Accuracy, absolute (including non-linearity, calibration uncertainty, repeatability) | ± 0.40% at 5% CO2 |
Temperature range | 5...60°C |
CO2-supply | max. 2 bar overpressure |
O2 sensor | zirconium oxide |
Measuring range | approximately 0% up
to ambient O2 (20.95% at dry air) |
Accuracy, absolute | ±0.40% at 5% O2 |
O2 sensor stability | ±0.5% full scale/year |
Setting, digital | ± 0.1% |
O2 control | PID, digital |
Temperature range | -10...80°C |
N2-supply | max. 0.5...0.8 bar overpressure |
110 - 120 V / 50-60 Hz | |
95 - 105 V / 50-60 Hz |
Tray size | EX (500 x 420mm) |
Loading, maximum | 25kg |
Setting, digital | 1 rpm |
Accuracy, absolute | ≤ 800 rpm = ± 0.5 rpm |
Timer | 1s ... 999h |
Acceleration | controlled |
Active brake | adjustable |
Stop on position | adjustable |
Speed | |
* orbital, Ø 12.5mm | 20...500 rpm |
* orbital, Ø 25.0mm | 20...400 rpm |
* orbital, Ø 50.0mm | 20...300 rpm |
* linear, ↔ 12.5mm | 20...400 rpm |
* linear, ↔ 25.0mm | 20...300 rpm |
* linear, ↔ 50.0 mm | 20…200 rpm |
* can be changed | |
Technical data subject to change
A simple and economical way of doubling the shaking capacity.
Touchscreen with Kuhner Insight Software for simple operation, monitoring and programming of incubator shakers.
Have you heard about our new EcoDew®-Technology? Find more information under kuhner technology.
Read more about our new O2 control option.
CO2 control is essential when working with mammalian cell cultures. Kuhner was the first company to manufacture and supply incubator shakers with CO2 control more than 15 years ago and continues to offer this option. More.
A reliable control of O2 is essential for cultivating cells (proliferation and metabolism of stem cells) and microorganisms with low/no oxygen demand. The O2 controller reduces the O2 level from ambient (20.95% at dry air; normoxia) to approximately 0% O2 (hypoxic). A digital PID controller adjusts the N2 flow rate to maintain the desired O2 value.
Visit our poster gallery and find
more information on the poster "Oxygen Transport Phenomena in Single-Use Shaking Flasks under Reduced Oxygen Conditions".pdf
All Kuhner incubator shakers can be fitted with cooling. The refrigeration system is regulated by a fully digital PID control ensuring minimal energy consumption. The cooling is switched on only when required as determined by the chamber temperature and set point. More.
Humidity control is an important factor in successful fermentation. Evaporation from microtiter plates, or when cultivating in flasks for long periods (e.g. cell cultures), can be significantly reduced with humidification.
To reduce evaporation from shake flasks or microtiter plates a water bath is placed inside the incubator. This water bath is fitted with an automatic water supply.
Our newly developed EcoDew®-Technology provides precise humidity control. More.
The ceiling of any Kuhner incubator shaker can be fitted with LED modules for the cultivation of phototrophic organisms. The control module allows full programming of night/day cycles and variable light intensity.
As in a biological safety cabinet, the chamber of a Kuhner incubator shaker can also be sterilized with an integrated UV lamp. The lamp is situated to maximise irradiation of the chamber and shaking table. The UV lamp has a clearly marked external switch and when the door is opened, the UV-lighting is automatically switched off.
Kuhner has designed a new light shade plate for the LT-X. This plate is easily placed in front of the door window and held in position with clamps. It prevents radiation affecting the contents of the incubator chamber and protects light sensitive media from UV, artificial and/or daylight.
Simply lift the light shade plate to look at the cultures without opening the incubator door. When light protection is no longer required, the plate is easily removed.
Check out the Kuhner EquipNote "Light protection for Kuhner incubators".
Available for light sensitive medium or organisms. Any Kuhner incubator shaker can be delivered with blackened windows to prevent unwanted daylight or UV radiation inside the incubator.
Check out the Kuhner EquipNote "Light protection for Kuhner incubators".
This option includes a 7-inch touchscreen with our Kuhner Insight Software. The touchscreen has been designed for simple operation, monitoring and programming. It can also be retrofitted to existing incubator shakers. More.
The dual table is an easy and economical way of doubling the shaking capacity. The dual shaking table consists of two levels. Each level will accept an E (420 x 420 mm) or EX (500 x 420 mm) size tray.
The dual table is available with a fixed distance between the two levels of either 120, 160, 180, or 200 mm. At the maximal distance of 200 mm the lower level accepts 125 mL, 250 mL, and 500 mL Erlenmeyer shake flasks.
However, with the dual table the shaking speed is limited to 200 rpm at a shaking diameter of 50 mm (all other maximum shaking frequencies, dependent on the shaking diameter, can be withdrawn from the LT-X manual).
Do you perform screenings with a large number of 50 ml bioreactors (TPP, Falcon etc.) or microtiter plates? Would you like to shake different cultivation vessels on the same shaker and be able to change the composition quickly and easily? Then the Kuhner EPFL-table is the optimal solution for you. The shaker table has 5 individual clamps, so that 5 different holders or EPFL universal trays can be clamped. By opening a clamp, a single holder or universal tray can be easily removed from the incubator shaker and processed under a clean bench while the remaining holders/trays keep on shaking.
The EPFL universal tray can be equipped with clamps for Erlenmeyer flasks, Schott laboratory bottles, test tube holders, microtiter plates or customer-specific holders. Rearrangement of these clamps and holders is quick and easy. The universal tray can also be equipped with sticky strips. Sticky strips are used to fix shake flasks or other equipment on shaking trays. They offer easy handling and flexible positioning of different sizes and shapes of shake flasks and other vessels.
Special holders for microtiter plates, 50 mL centrifuge tubes (Falcons) and 600 mL reaction vessels are also available for the EPFL-table.
Instead of 5 individual holders, a large Kuhner F-tray (800 x 420mm) can also be clamped onto the shaker table.
For the smaller (incubator) shakers from Kuhner, correspondingly adapted EPFL-tables with 3 or 4 clamps are available (LT-X: 3 clamps, LS-Z: 3 or 4 clamps).
EPFL-table
EPFL-table with different holders
F-size tray clamped on the EPFL-table
Clamp handle
Order number | Description |
|---|---|
100125 | Holder for 24 x 50 ml Falcon/TPP tubes mounted on a base plate |
Order number | Description |
|---|---|
104173 | Cover clamp for 4 deepwell MTPs (Duetz) |
Order number | Description |
|---|---|
104104 | Universal clamp for 12-16 lowwell MTPs or 4 deepwell MTPs (Duetz) |
Order number | Description |
|---|---|
107322 | EPFL-Tray Universal kpl. can be fitted with clamps |
Order number | Description |
|---|---|
105186 | Holder 5x600mL TPP tubes, straight tubes |
108226 | Tube set for holder 5 x TPP 450ml |
Order number | Description |
|---|---|
100633 | Holder for 3 x 600ml reactor |
Kuhner TOM (Transfer-Rate Online Measurement) measures online oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR) and the respiratory quotient (RQ) in up to 16 individual shake flasks for better process understanding and informed process development. TOM is easily installed in any Kuhner shaker and can be operated with various flask types and sizes including single use shake flasks. Kuhner TOM has zero footprint on the shaker tray as it is mounted above (see picture below). More.
AerationSystem and FlowCon provide the ideal system for carrying out cell/stem cell cultivations in a simple, fast, and inexpensive manner.
Lab shakers in
temperature-controlled rooms and incubator shakers without humidity and CO2-control
can be equipped with the AerationSystem and FlowCon to easily implement humidification and a controlled
gas composition for carrying out cell cultivations (e.g., CHO, HEK cells…) in
shake flasks.
The FlowCon is a stand-alone gas mixing device and provides a precise gas composition and flow rate. Simultaneously, the AerationSystem (consisting of GasDivider and special shake flask caps) humidifies and distributes the gas flow evenly to the connected shake flasks. Evaporation losses from the shake flasks are significantly reduced, which is particularly advantageous for very long cultivation times (such as cell culture processes) and contributes to successful cultivations. Another advantage is that opening the incubator door does not affect the gas composition in the shake flasks when they are connected to the aeration system.
By controlling the composition of the inlet air with the FlowCon, it is not only possible to supplement CO2 but also to increase or reduce the concentration of oxygen (O2) or implement anoxic conditions using nitrogen (N2). This control of gas compositions allows for a variety of different applications, including stem cell processes for gene therapy or cultivated meat that require a physiological oxygen concentration at 5% O2 or anaerobic cultivations in a nitrogen atmosphere.
The patented Kuhner GasDivider humidifies the mixed, dry gas flow provided by the FlowCon and distributes it precisely and evenly across the attached shake flasks, independent of shake flask type, tube length and any filters in-between. As the GasDivider is placed on the shaking tray of the (incubator) shaker, the gas flow is also perfectly adapted to the temperature of the incubator shaker or temperature-controlled room.
To prevent accidental operation of the controllers, the panel can be protected by a perspex cover.
The TabCom option from Kuhner consists of a cable for power and data with the connection port integrated in the shaking table (see picture below). A cable guide prevents the cable breaking and ensures secure data recording. The flexibility of TabCom means online measurement systems can be easily integrated.
IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.
The universal tray can be fitted with clamps for Erlenmeyer flasks, test tube holders, microtiter plates, or custom made holders to suit your requirements.
Rearranging these clamps and holders is quick and simple.
Order number | Description |
|---|---|
102201 | Universal tray EU (420 x 420 mm) |
102207 | Universal tray EXU (500 x 420 mm) |
The clamps are riveted onto these trays and cannot be removed. They offer maximum stability and will often hold a larger number of flasks than universal trays. These trays are available in a variety of flask clamp sizes from 25 to 6000ml.
Ordner number | Description | Number of clamps |
|---|---|---|
101525 | E-25 ml | 81 |
101526 | E-50 ml | 50 |
101527 | E-100 ml | 39 |
101528 | E-125 ml | 30 |
101529 | E-150 ml | 30 |
101530 | E-200 ml | 20 |
101531 | E-250 ml | 18 |
101532 | E-300 ml | 15 |
101533 | E-500 ml | 12 |
101534 | E-1000 ml | 9 |
101535 | E-1500 ml | 5 |
101536 | E-2000 ml | 5 |
101537 | E-3000 ml | 4 |
101538 | E-4000 ml | 2 |
101539 | E-5000 ml | 2 |
101540 | E-6000 ml | 1 |
Order number | Description | Number of clamps |
|---|---|---|
101541 | EX-25 ml | 90 |
101542 | EX-50 ml | 60 |
101543 | EX-100 ml | 42 |
101544 | EX-125 ml | 36 |
101545 | EX-150 ml | 32 |
101546 | EX-200 ml | 25 |
101547 | EX-250 ml | 21 |
101548 | EX-300 ml | 18 |
101549 | EX-500 ml | 14 |
101550 | EX-1000 ml | 9 |
101551 | EX-1500 ml | 8 |
101552 | EX-2000 ml | 5 |
101553 | EX-3000 ml | 4 |
101554 | EX-4000 ml | 3 |
101555 | EX-5000 ml | 3 |
101556 | EX-6000 ml | 2 |
Kuhner has designed trays specifically for microtiter plates. Handles at the front of the tray open and close the special clamping mechanism allowing plates to be loaded and unloaded. Trays of different heights (22, 47 and 77mm) allow the stacking of up to four microtiter plates. The trays are available in three sizes and suitable for all types of plates (deep well, low well, 24, 48 and 96 well).
Order number | Description | Numbers of clamps |
|---|---|---|
104947 | E-MT.22 | 12 to 24 |
104950 | E-MT.47 | 12 to 48 |
104948 | E-MT.77 | 12 to 72 |
Kuhner has designed a new tray for microtiter plates. This tray is fitted with springs which allows each well plate to be loaded and unloaded individually. The tray is available in three sizes: E (420x420mm), EX (500x420mm) and F (800x420mm).
This new tray is particularly suitable for the special holder with filter lid for deep well microtiter plates.
Order number | Description | Number of mircotiter plates |
|---|---|---|
104806 | E-tray(420 x 420 mm) for deepwell or mircrotiter plates | 1-12 |
104794 | EX-tray (500 x 420 mm) for deepwell or mircotiter plates | 1-15 |
The whole tray is covered with a rubber mat and is particularly suitable for shaking containers at low speed (e.g. blood bags).
Order number | Description |
|---|---|
105312 | Rubber mat EG |
105313 | Rubber mat EXG |
A tray fitted with sticky strips is especially useful for flasks shaken at low speeds (up to 200 rpm). The flask is placed directly on the sticky strip without the need for any other support. Flasks of all sizes can be used. Sticky strips are available individually or in sets and can also be supplied already fitted to a tray. The EX-size tray accepts five strips.
Order number | Description |
|---|---|
105169 | E-tray with 4 sticky strips (clear) |
105199 | EX-tray with 5 sticky strips (clear) |
105194 | 1 sticky strip: 385 x 85 x 3 mm (clear) |
The PC-plate increases the adhesion of the sticky strips. This is particularly helpful if using flasks >500ml.
Order number | Description |
|---|---|
105224 | EX-tray with 5 sticky strips (clear) |
105194 | 1 sticky strip: 385 x 85 x 3 mm (clear) |
105196 | 1 big sticky strip: 395 x 385 x 3 mm (clear) |
The dual tray offers two shaking levels and doubles the capacity. It is available in EXU, EX, FU and F size and the upper tray can be removed.
Please note the shaking speed is limited.
Order number | Description |
|---|---|
100548 | two EXU trays with 200mm distance (with holes) |
102226 | two EX trays with 200mm distance |
These trays offer an alternative to both clamps and sticky mats and are ideal for tall containers. The tray is covered with a rubber mat and has an upright at each corner. The EX size tray is fitted with four cross supports. These support bars securely clamp containers.
Order number | Description | Number of longitudinal girders |
|---|---|---|
106408 | EA-tray with rubber mat and 4 cross supports | 2 |
106407 | EXA- tray with rubber mat and 4 cross supports | 2 |
Kuhner has also designed a special tray for shaking separating vessels. This very flexible tray can take vessels of various sizes. It is also possible to add a second layer and double the capacity. Movable retaining rods allow vessels to be installed easily.
Order number | Description | Number of separating vessels |
|---|---|---|
SM420250 | ES-250 | 5 |
SM4202502 | ES-250/2 | 10 (2 layers) |
SM420500 | ES-500 | 4 |
SM4205002 | ES-500/2 | 8 (2 layers) |
SM421000 | ES-1000 | 3 |
SM4210002 | ES-1000/2 | 6 (2 layers) |
The pin-clamps are ideal for cleanrooms and areas where sticky mats or other standard clamps are not suitable. The clamps have been designed for easy cleaning and efficient handling of samples. The stainless-steel pin-clamps are compatible with all Kuhner universal trays. The shaking speed is limited to a maximum of 180 rpm at 50 mm shaking diameter.
| Order number | Description |
|---|---|
| 107167 | Pin-clamp 1000 mL Schott bottle/ 500 mL Erlenmeyer flask |
| 107168 | Pin-clamp 500 mL Schott bottle |
| 107324 | Pin-clamp 250 mL Erlenmeyer flask |
| 107326 | Pin-clamp 125 mL Erlenmeyer flask |
| 108729 | Pin-clamp 1000 mL Erlenmeyer flask |
| 108730 | Pin-clamp 1000 mL Duran / Kuhner TOM |
| 108731 | Pin-clamp 1600 mL Thomson Optimum Growth |
| 108732 | Pin-clamp 2800 mL Thomson Optimum Growth |
| 108733 | Pin-clamp 2000 mL Erlenmeyer flask |
| 108734 | Pin-clamp 3000 mL/ 5000 mL Fernbach flask / Corning & Thomson Optimum Growth |
For a comfortable working height Kuhner offers floor stands for both the ISF1-Z and LT-X incubator shakers. These are available in a choice of 400mm (2 x LT-X) or 765mm (1 x LT-X) high.
Order number | Description |
|---|---|
100834 | 400mm high for 2 x LT-X |
100838 | 765mm high for 1 x LT-X |
Floor stands with wheels are available for both the ISF1-Z and LT-X incubator shakers. These stands enable the user to move the machines easily. However, the shaking speed is limited.
Order number | Description |
|---|---|
100842 | 400mm high for 2 x LT-X |
100841 | 765mm high for 1 x LT-X |
105008 | Upgrade costs for castors (Polyamid)* |
*only for new machines | |
This stacking adapter is designed for stacking a LT-X on an ISF1-X/Z incubator shaker. Extra work space on the adapter allows room for flasks or a small machine, as well as the LT-X, thus creating a greater working area.
Order number | Description |
|---|---|
105864 | Stacking adapter (LT-X on ISF1-X/Z) |
The user-friendly Kuhner Insight software was developed for supervision, monitoring, and recording of data. The software is easy to operate. More.
Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces. More.
The Kuhner LT-X incubator shaker can be fitted with a shelf allowing cultivation in petri dishes. One or two shelves can be placed above the shaking table and fixed to the chamber walls with four screws.
Order number | Description |
|---|---|
102017 | Shelf for LT-X |
To reduce evaporation from shake flasks or microtitre plates a stainless steel water bath can be placed inside the incubator. This water bath is not fitted with an automatic water supply and must be topped up manually.
Order number | Description |
|---|---|
100799 | Humidity bath, coated, for LT-X |
Kuhner has designed a new light shade plate for the LT-X. This plate is easily placed in front of the door window and held in position with clamps. It prevents radiation affecting the contents of the incubator chamber and protects light sensitive media from UV, artificial and/or daylight.
Simply lift the light shade plate to look at the cultures without opening the incubator door. When light protection is no longer required, the plate is easily removed.
Check out the Kuhner EquipNote "Light protection for Kuhner incubators".
Order number | Description |
|---|---|
100855 | Light shade plate for LT-X |
Monitoring of pH and OTR using a multiple shake flask platform
Characterization of Algal Cell Culture in Photobioreactors
Oxygen Transport Phenomena in Single-Use Shaking Flasks
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
Yes
No, only two.
Yes, using a special fitting.
Yes, an EX-tray has space for two 6 litre Erlenmeyer flasks. |
Yes |
Yes
The following flyers provide information about the machines and function-specific solutions.
Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.
Adolf Kühner AG (Headquarters)
Dinkelbergstrasse 1
CH-4127 Birsfelden (Basel)
Switzerland
We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
Read more
The world's most reliable shaking machines built in-house in Switzerland.
