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The SB50-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 15 litres up to 50 litres for the SB50-X ensures easy scale-up from microtiter plates to shaking flasks and all the way up to 200 litres. The SB50-X can be used in research, process development or production.
The SB50-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).
Large capacity (50L working volume)
single use bioreactor system
Cultivation of human, mammalian and plant cells
Fast, simple set up
Easy scale-up
Online measurement of pH and DO
5 year warranty
Single-Use
Scale-Up
GMP
Shaking diameter
Dr. Shakers Helpful Hint N°4 explains what the shaking diameter
is and how you can determine the shaking diameter of your lab shaker.
Find information on the importance of the shaking diameter for biological cultivations in our Shaking Technology Forum: Why is the shaking diameter important?
The cylindrical vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
The orbital motion of the SB50-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB50-X uses the unique Kuhner direct drive which provides accurate control of the orbital speed.
The SB50-X fills a gap in the scale-up chain from microtiter plates all the way up to 200 litres. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range. The culture from the SB50-X can also serve as preculture or inoculum for the SB200-X.
The bespoke control unit, designed by Kuhner, uses the latest touch-screen
technology and Kuhner Insight software for data recording, calibration,
programming and controlling. Technical controls for 21 CFR Part 11 compliance
are built into the software.
The SB50-X can be heated or cooled. Temperature ramps are easily programmed and
processed.
Online measurement of each parameter (dissolved oxygen and pH-value in the
medium) is available.
The control unit incorporates two small and one big peristaltic pump.
This enables simple regulation of the pH value, feeding and the transfer of the
culture media (scale-up or processing).
The SB50-X has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen
concentrations, the gas mixer meets all user needs. Four mass flow controllers
ensure highly accurate and reproducible gas mixing. An integrated pressure
sensor prevents over pressure in the bioreactor by reducing the gas flow in the
first step (1. limit) and shutting off the gas flow in the second step (2.
limit). Step limits can be freely programmed by the user. Integrated air
filters and back pressure valves ensure interference free and long operating
times for the FlowCon.
A height adjustable filter heater, with up to two exhaust filters, is
installed above the cylindrical vessel. Special emphasis has been placed on
developing a user friendly unit. The filter heater combats any water vapour
condensation in the outlet air which might cause the membranes to block. The
temperature is monitored and controlled by Kuhner Insight software.
We subject all our shaking machines to an elaborate final test before delivery and send the test protocols to our customers.
Shaker speed | max. 150 rpm |
Shaker diameter | 50 mm (orbital motion) |
Weight | approx. 340 kg without liquid |
Accuracy, absolute | ± 0.5 rpm |
Setting, digital | 1 rpm |
Active brake | adjustable |
Interface | CAN-Bus, RS232 |
Temperature | 5°C - 40°C |
Cooling | cooling coils are incorporated for connection to an external cooling system (pressure < 0.2bar) |
Working volume | 15 L - 50 L |
Technical data subject to change | |
105327 | 220 - 240 V / 50-60 Hz |
102023 | 110 - 120 V / 50-60 Hz |
pH measurement | principle: optical chemosensor |
Range | pH 5.5 – pH 8.5 |
Accuracy (chemosensor) |
± pH 0.05 at pH 7 with one point calibration ± pH 0.10 at pH 7 with pre-calibration |
Drift | < pH 0.005 per day |
Temperature range | up to 50°C |
| |
DO measurement | principle: optical chemosensor |
Range | 0% – 100% DO |
Accuracy (chemosensor) | ± 0.1% O2 at 20.9% O2 |
Accuracy (system) | ± 10% DO |
Drift | < 0.015% O2 per day |
Temperature range | up to 50°C |
Capacity | 2 exhaust filters |
Temperature range |
Ambient temperature up to 45°C, monitored and controlled by Kuhner Insight software |
| 101777 |
Weight | 120kg |
Monitor | 15'' touchscreen monitor |
Software | for monitoring, calibration, controlling |
Monitoring | O2, pH, shaking
speed, temperature |
Calibration | pre-calibrated
sensors in bag |
Recalibration | one point
recalibration is possible |
Applicable | XP/Vista/Win7&8 |
GMP | 21 CFR Part 11
compliance (audit trail) |
2 small pumps (A+B) | Watson-Marlow 313VBM/D |
Gear | 10 |
Maximum Speed | 100 rpm |
Power | 70 W |
Rollers | 3 |
Tube | 1.6 mm wall tubing |
Delivery Pressure | max. 2bar |
1.6 mm wall
tubing | Flow rates
(ml/min at 100 rpm) |
0.5 | 3.4 |
0.8 | 7 |
1.6 | 27 |
3.2 | 100 |
4.8 | 220 |
6.4 | 360 |
8.0 | 500 |
1 big pump (C) | Watson-Marlow 520VBM/R2 |
Gear | 10 |
Maximum Speed | 400 rpm |
Power | 70 W |
Rollers | 2 |
Tube | 2.4 mm wall tubing |
Delivery Pressure | max. 2bar |
2.4 mm wall tubing | Flow rates (ml7min at 100rpm) |
0.5 | 4.1 |
0.8 | 10.5 |
1.6 | 42 |
3.2 | 170 |
4.8 | 380 |
6.4 | 680 |
8.0 | 1040 |
9.6 | 1500 |
Number of mass flow controllers | up to 4 |
Ambient conditions | 5-40 [°C], max. 85 [%rH] |
Input |
up to 4 gases, push
in connector 6 [mm] |
Input pressure | 1-2 bar |
Output | 1, (tube fitting for
tube ID 13 [mm]) |
Flow rate | 0 - 20 [sL/min] |
Accuracy | ±1.5% from the end value |
Pressure sensor |
integrated, 0-400
[mbar], programmable |
Air filter | integrated, prevents
blocking the mass flow controllers |
Back pressure valve | integrated, prevents
reflux into the mass flow controllers |
Controlled filter | for the outlet filters,
prevents blocking of the filters caused by condensation |
C-Flex material | for the tubing,
enables the user to connect additional components via tube welding |
Technical data subject to change
Would you like to aerate other one-way systems? Please use our stand-alone device.
The SB50-X has a cooling ring embedded in the base plate. Cooling is achieved by connecting an external cooling device. An integrated valve, controlled by the Software, regulates the set point temperature.
A tubing support bar is available for attaching the various tubes (feeding, medium, air and pH regulation) and keeping them tidy.
IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification required for GMP procedures. Documentation is available from Kuhner and Qualification services can also be provided at the customer`s premises.
The gamma irradiated bag for the Kuhner shaken bioreactor requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. The single-use bag has a working volume from 15 litres up to 50 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
Order number | Description |
|---|---|
105330 | Bag for SB50-X with optical sensors |
Order number | Description |
|---|---|
SMX870001 | Red
overpressure valve, OD 14mm |
106490 | Glass
fibers for pH/DO probes, length 1.5m |
Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces. More.
Orbital Mixing as an Alternative to Stirred-Tank Bioreactors
Characterization of orbitally shaken single-use bioreactors
Orbital Shaken Bioreactors
The Scale Up Fast Track
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
Yes, no other equipment is required.
No, the single-use bag is simply placed in the SB50-X vessel, positioned and then inflated.
No, shaken systems have a very homogenous and low local power input. This means that high local power inputs which are responsible for mechanical stress, simply don’t occur.
Yes
Yes
The following flyers provide information about the machines and function-specific solutions.
Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.
Adolf Kühner AG (Headquarters)
Dinkelbergstrasse 1
CH-4127 Birsfelden (Basel)
Switzerland
We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
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