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SB2500-Z single-use bioreactor

SB2500-Z single-use bioreactor
Technical Data

Orbital shaken bioreactor for single-use bags with a working volume up to 2500 litres

The next generation of manufacturing-scale orbital shaken bioreactor (OSB) SB2500-Z is now available for the cultivation of human, mammalian, plant, and insect cells in a single-use bag. The bioreactor system has a working volume of 500 to 2500 liters. This user-friendly system has comparatively short set-up times and can be easily deployed in production. The SB2500-Z complies with GMP requirements and regulations.

Performance characteristics

  • Large range of working volume: 500-2500 L
  • Cultivation of human, mammalian, plant, and insect cells
  • User-friendly & fast and simple set-up
  • 3D single-use bag
  • Online measurement and control of pH and DO
  • Qualification for GMP production processes
Single-use bag

Single-use bag





More informationen

Shaking diameter

Dr. Shakers Helpful Hint N°4 explains what the shaking diameter is and how you can determine the shaking diameter of your lab shaker.

Dr. Shaker

Find information on the importance of the shaking diameter for biological cultivations in our Shaking Technology Forum: Why is the shaking diameter important?

Application areas in production

  • Mammalian/human cells (e.g., antibodies, vaccines)
  • Stem Cells (e.g., processed meat)
  • Plant Cells (e.g., antibodies, food supplements)
  • Insect Cells (e.g., antibodies, vaccines)

Single-use bag for a fast and simple set up

The gamma irradiated 3D single-use bag for the Kuhner orbital shaken bioreactor requires no additional mixing device and is therefore light and easy to handle. This enables quick set up times and eliminates elaborate cleaning and sterilizing procedures. The single-use bag has a working volume from 500 liters up to 2500 liters. Various ports are incorporated to allow inoculation, feeding, sampling, pH regulation, and harvesting. Two optical sensors each for pH and for DO measurement are built in. Customized bags are easily available on request.

Orbital motion

The orbital motion of the SB2500-Z ensures efficient liquid mixing with low localized power input per unit volume. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing.

Trouble free scale-up

Another advantage of the SB2500-Z is the easy scale-up from microtiter plates over shake flasks to shaken bioreactors all the way up to 2500 liters. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the scaling process.

Good Manufacturing Practice (GMP) and IQ/OQ Documentation

The SB2500-Z complies with GMP requirements and regulations. Kuhner products have established a global reputation of excellence under GMP requirements. Documentation, development and production along with the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

IQ-OQ (Installation Qualification and Operation Qualification), an equipment qualification required for GMP procedures, is available from Kuhner and qualification services can also be provided at the customer's premises.

We subject all our shaking machines to an elaborate final test before delivery and send the test protocols to our customers.

Reliable and monitored drive system

The shaking unit of the SB2500-Z uses a robust and long-lasting drive system which guarantees accurate control of the orbital speed and low energy consumption. The drive system (agitation) requires zero bag penetrations and runs without belts, meaning no abrasion or particle generation, an important factor for production processes in cleanrooms. Motor-specific parameters are constantly monitored, allowing for predictive maintenance and failure prevention. The entire drive unit may be exchanged with a process in place in less than 2 hours.

Control unit

The bespoke control unit, designed by Kuhner, uses the latest touch-screen technology and Siemens SCADA for data recording, calibration, programming and controlling. Technical controls for 21 CFR Part 11 compliance are built into the software.

Temperature regulation

The SB2500-Z can be electrically heated or (optionally) cooled. If cooling is chosen as an option, a cooling ring is embedded in the base plate of the SB2500-Z. Cooling is achieved by connecting an external cooling device. An integrated valve, controlled by the software, regulates the set point temperature. Temperature ramps are easily programmed and processed.

Online measurement and control of pH and DO (dissolved oxygen)

The single use bag has built in pre-calibrated, optical sensors for pH and DO (two of each). The duplex online measurement of both parameters in the medium ensures greater process safety. The pH value can be controlled by the addition of acid/base via peristaltic pumps or stabilized via dosage of CO2. To control the DO, the gas flow of the inlet gas can be increased or decreased, the percentage of oxygen in the inlet gas can be varied (via the gas mixing device), and the shaker speed can be adjusted.

Pumping system

There are three peristaltic pumps, which can be placed external, mobile and can be controlled by the bioreactor software. The control unit incorporates two smaller and one large peristaltic pump. This enables simple regulation of the pH value, feeding and the transfer of the culture media for downstream processing.

Gas mixing (FlowCon)

The SB2500-Z has an integrated gas mixing device (FlowCon). Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor by reducing the gas flow in the first step and shutting off the gas flow in the second step. Step limits can be freely programmed by the user. There is also another pressure sensor directly connected to the bag via the filter. Integrated gas filters ensure interference free and long operating times for the FlowCon.

Filter heater

A height adjustable filter heater, with up to two exhaust filters, is installed above the vessel. Special emphasis has been placed on developing a user-friendly unit. The filter heater combats any water vapor condensation in the outlet air which might cause the membranes to block. The temperature is monitored and controlled by the Siemens SCADA software of the control unit.


Product name


 Order number



Working volume

500 L - 2500 L


(width x depth x height)

approx. 220 x 220 x 410 cm

Required footprint bioreactor

5 m2

Required footprint operation site

12 m2

(bioreactor incl. shaker

approx. 3200 kg without liquid

Power consumption steady state

approx. 1000 Wh

Power consumption max.

approx. 11500 Wh

Mains connection

400 V / 50 Hz

Connection for power supply

Adjusted to power outlet of recipient country


Ethernet (1x RJ45)

Language of manual and safety instructions

Adjusted to official language of recipient country

Ambient conditions

10 - 35 °C / max. 85% r.h.

Material casing

Stainless steel V2A / 1.4301

IP protection class


Ambient noise level

approx. 60 dB

Bag nominal value

approx. 3700 L

Bag contact layer


Technical data subject to change

User interface

Touchscreen size

21.5 inches / 54.6 cm

Touchscreen type

10-finger multi-touch, glove-friendly, capacitive


1920 x 1080 Pixel (Full-HD)

Material casing

stainless steel & safety glass

IP protection class


Control software


Operation menu language


Shaking control

Drive concept

Helical-bevel gearmotor

Shaking frequency range

0 - 60 rpm

Setting, digital

1 rpm

Shaking diameter (fix)

100 mm (orbital motion)

Accuracy, absolute

± 1 rpm



Active brake


Shaking frequency sensor

inductive sensor for additional speed measurement and detection of drivetrain defect

Dimensions (in mm) shaken bioreactor


Temperature control



Temperature max.  

(with 2500 L)

40 °C

Temperature min. 

(with 2500 L)

slightly above RT (with optional cooling)

Setting, digital

0.1 °C

Temperature control accuracy

± 0.3 °C

Heating rate

(with 2500 L)

2 °C / h

Cooling rate 

(with 2500 L)

1 °C / h (with active coolant supply at 16 °C)

Power of heating

9000 W

Heating concept

resistance (attached to vessel wall)

Power of cooling

7500 W (depending on available coolant supply)

Cooling concept

Cooling coils in vessel bottom (requires an external coolant supply)

Temperature sensors

4 x Pt-100 Class B3 (integrated in vessel bottom)

pH and DO control

pH measurement

2 x optical sensors (integrated in bag)

Measurement range

pH 5.0 - 9.0

Measurement accuracy

pH ± 0.05 at pH 7 with one point calibration

pH ± 0.10 at pH 7 with pre-calibration

Control range

pH 5.5 - 8.5

Control accuracy

pH ± 0.1

Drift per day

pH < 0.005

Setting, digital

pH 0.1

Temperature range

up to 50 °C

Control strategy

variable CO2 conc. in gas mixture and/or acid/base pumps

DO measurement

2 x optical sensors (integrated in bag)

Measurement range

0% – 130 % DO

Measurement accuracy

+/- 0.4 % O2 at 20.9 % O2 in the gas mixture 

+/- 0.05 % O2 at 0.2 % O2 in the gas mixture

Control range

0% – 100% DO

Control accuracy

± 1 % DO

Drift per day

< 0.015 % DO

Temperature range

up to 50 °C

Control strategy

variable O2 conc. in gas mixture

Gas mixing device (FlowCon)

Number of mass flow controllers

5 (1 per gas and 1 for total gas flow)

Mass flow controller concept



up to 4 gases (air, O2 , N2 and CO2 )

Input pressure range

2.0 - 2.5 bar

Output flow rate control range air for bag infilling

1.0 - 200 L/min

Output flow rate control range air for process control

1.0 - 70 L/min

Output flow rate control range O2 for process control

0.8 - 60 L/min

Output flow rate control range N2 for process control

0.8 - 60 L/min

Output flow rate control range CO2 for process control

0.1 - 15 L/min

Flow rate accuracy of full scale

± 0.8 %

Setting, digital

0.1 L/min

Pressure safety measurement

in gas output flow and bag headspace

Filter heater control

Exhaust filter capacity

2 (2 separate filter heaters)

Temperature maximum (at RT with 60 L/min flow rate)

60 °C

Heating concept


Power of heating per filter heater

55 W

Setting, digital

0.1 °C

Control accuracy

± 2 °C

Temperature sensors

2 x Pt-100 Class B4

Material casing

water repellent polyester fabric


 Peristaltic pumps 

(up to 3 mobile pumps)

2 small pumps for acid, base, or feed

1 large pump for inoculation and harvest


Watson-Marlow 630PnN/R

Watson-Marlow 730PnN/R

Flow rates

0.010 L/min to 2.400 L/min

1.000 L/min to 33.330 L/min

Tube wall thickness

3.2 mm

4.8 mm

Flow measurement

By internal measurement of pumps and additional up to 3 flow meters (Leviflow®)

Technical data subject to change

The following flyers provide information about the machines and function-specific solutions.

Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.

Stem cells
Cell culture
Plant cells & algae


Adolf Kühner AG (Headquarters)

Dinkelbergstrasse 1

CH-4127 Birsfelden (Basel)



We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently. 

GMP compliant production of shakers and bioreactors

For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

Read more


The world's most reliable shaking machines built in-house in Switzerland.

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