Select a different language or country,
to view content for your location.


14 Apr, 2021
Kuhner Switzerland (Headquarters)

OTR measurement with Kuhner TOM paves the way…

Media optimization for microbial induced calcite precipitation with OTR measurement in the Kuhner TOM.

OTR measurement with Kuhner TOM paves the way…

Frédéric Lapierre and Robert Huber from Munich University of Applied Science recently published their article on the nutritional requirements of Sporosarcina pasteurii for microbial-induced calcite precipitation (MICP). The bacterium enables calcite precipitation by ureolysis which can reinforce soil (e.g. pavement of sandy roads) or concrete. Large amounts of biomass are needed at a low price to make these processes feasible. Consequently, the researchers looked for supplementing complex media to boost biomass yield.

Primary screening of nutrients in microplates was followed by comparing modified and non-modified media in shake flask cultures. On modified medium, monitoring of OTR with the Kuhner TOM revealed a much longer growth phase, but also oxygen and secondary substrate limitations, a possibility for further enhancement. In total, the biomass yield was improved by approximately 400%.

See the publication at (Lapierre FM et al. Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media. Sci Rep . 2020;10(1):1–15.


«Kuhner respects your privacy»

We use cookies to provide you with an innovative experience on our website and to improve our communication with you. Cookies on this website are used to ① improve the user experience (strictly necessary Cookies) ② personalize information about your country and ③ learn how our website is used.

close x

Necessary for the usability of the website (always active)

Necessary cookies help to make a website usable by enabling basic functions such as page navigation and access to secure areas of the website. The website cannot function properly without these cookies.

Save country and language settings for convenience 

Country and language cookies allow our website to remember information such as: Your preferred language or country of residence. You will always receive the right information and valid offers tailored to your country.

Statistics for performance measurement 

Statistics cookies help us as a website operator to understand how visitors interact with websites by collecting and reporting information anonymously. From the statistics, we gain insights in order to constantly adapt the further development of our communication to the needs of the visitors.

Further information on the handling of cookies and information on data protection can be found here. You can adjust your cookie preferences in the settings.