A novel publication was recently released demonstrating the advantages of the Kuhner Feeding Technology in strain selection.
The scientific work was performed by the RWTH Aachen University in collaboration with BASF. It demonstrates how a catabolite repressed strain performs comparably better between screening in microtiter plates and cultivation in lab-scale stirred fermenters (1 L) if fed-batch screening conditions are applied. The developed glucose-limited fed-batch screening procedure was applied to 13 industrially relevant clones from two B. licheniformis strains. When screening under fed-batch conditions, 12 out of 13 clones (92%) were classified similarly to a lab-scale fed-batch fermenter process operated under glucose-limited conditions. When the microtiter plate was running in batch mode, only 5 out of 13 clones (38%) were classified similarly as in the lab-scale fed-batch fermenter process. This result notably highlights the potential of improving screening conditions for our customers.
See the publication: https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-021-01541-2
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