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28 Apr, 2021
Kuhner Switzerland (Headquarters)

New publication about substrate-limited strain screening with the Kuhner FeedPlates®

A novel publication was recently released demonstrating the advantages of the Kuhner Feeding Technology in strain selection.

New publication about substrate-limited strain screening with the Kuhner FeedPlates®

The scientific work was performed by the RWTH Aachen University in collaboration with BASF. It demonstrates how a catabolite repressed strain performs comparably better between screening in microtiter plates and cultivation in lab-scale stirred fermenters (1 L) if fed-batch screening conditions are applied. The developed glucose-limited fed-batch screening procedure was applied to 13 industrially relevant clones from two B. licheniformis strains. When screening under fed-batch conditions, 12 out of 13 clones (92%) were classified similarly to a lab-scale fed-batch fermenter process operated under glucose-limited conditions. When the microtiter plate was running in batch mode, only 5 out of 13 clones (38%) were classified similarly as in the lab-scale fed-batch fermenter process. This result notably highlights the potential of improving screening conditions for our customers.

See the publication:

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