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The SB200-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 50 litres up to 200 litres for the SB200-X ensures easy scale-up from microtiter plates to shaking asks and all the way up to 200 litres. This user friendly system can be easily deployed in research, process development or production.
The SB200-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).
5 year warranty
Single-Use
Scale-Up
GMP
Shaking diameter
Dr. Shakers Helpful Hint N°4 explains what the shaking diameter
is and how you can determine the shaking diameter of your lab shaker.
Find information on the importance of the shaking diameter for biological cultivations in our Shaking Technology Forum: Why is the shaking diameter important?
The vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
The orbital motion of the SB200-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB200-X uses the unique Kuhner direct drive which provides acurate control of the orbital speed.
Another advantage of the SB200-X is easy scale-up from microtiter plates all the way up to 200 litres. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range.
The bespoke control unit, designed by Kuhner, uses the
latest touch-screen technology and Kuhner Insight software for data recording,
calibration, programming and controlling. Technical controls for 21 CFR Part 11
compliance are built into the software.
The SB200-X can be heated or cooled. Temperature ramps are easily programmed and
processed.
Duplex online measurement of each parameter (dissolved oxygen and pH value)
is available ensuring greater process safety.
The control unit incorporates two small and one big peristaltic pump. This enables simple regulation of the pH value, feeding and the transfer of the culture media (scale-up or processing).
The SB200-X has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen
concentrations, the gas mixer meets all user needs. Four mass flow controllers
ensure highly accurate and reproducible gas mixing. An integrated pressure
sensor prevents over pressure in the bioreactor by reducing the gas flow in the
first step (1. limit) and shutting off the gas flow in the second step (2.
limit). Step limits can be freely programmed by the user. Integrated air
filters and back pressure valves ensure interference free and long operating
times for the FlowCon.
A height adjustable filter heater, with up to two exhaust filters, is installed above the vessel. Special emphasis has been placed on developing a user friendly unit. The filter heater combats any water vapour condensation in the outlet air which might cause the membranes to block. The temperature is monitored and controlled by Kuhner Insight software.
We subject all our shaking machines to an elaborate final test before delivery and send the test protocols to our customers.
Shaker speed | max. 80 rpm |
Shaker diameter | 50 mm (orbital motion) |
Weight | approx. 400 kg without liquid |
Accuracy, absolute | ± 0.5 rpm |
Setting, digital | 1 rpm |
Active brake | adjustable |
Interface | CAN-Bus, RS232 |
Temperature | 5°C - 40°C |
Cooling | cooling coils are incorporated for connection to an external cooling system (pressure < 0.2bar) |
Working volume | 50 L - 200 L |
Technical data subject to change | |
102023 | 110 - 120 V / 50-60 Hz |
102024 | 95 - 105 V / 50-60 Hz |
pH measurement | principle: optical chemosensor |
Range | pH 5.5 – pH 8.5 |
Accuracy (chemosensor) |
± pH 0.05 at pH 7 with one point calibration ± pH 0.10 at pH 7 with pre-calibration |
Drift | < pH 0.005 per day |
Temperature range | up to 50°C |
| |
DO measurement | principle: optical chemosensor |
Range | 0% – 100% DO |
Accuracy (chemosensor) | ± 0.1% O2 at 20.9% O2 |
Accuracy (system) | ± 10% DO |
Drift | < 0.015% O2 per day |
Temperature range | up to 50°C |
Capacity | 2 exhaust filters |
Temperature range | Ambient temperature up to 45°C, |
105824 | |
Weight | 120kg |
Monitor | 15'' touchscreen monitor |
Software | for monitoring, calibration, controlling |
Monitoring | O2, pH, shaking speed, temperature |
Calibration | pre-calibrated
sensors in bag |
Recalibration | one point recalibration is possible |
Applicable | XP/Vista/Win7&8 |
GMP | 21 CFR Part 11 compliance
(audit trail) |
2 small pumps (A+B) | Watson-Marlow 313VBM/D |
Gear | 10 |
Maximum Speed | 100 rpm |
Power | 70 W |
Rollers | 3 |
Tube | 1.6 mm wall tubing |
Delivery Pressure | max. 2bar |
| 1.6 mm wall tubing Bore diameter (mm) | Flow rates (ml/min at 100 rpm) |
0.5 | 3.4 |
0.8 | 7 |
1.6 | 27 |
3.2 | 100 |
4.8 | 220 |
6.4 | 360 |
8.0 | 500 |
1 big pump (C) | Watson-Marlow 520VBM/R2 |
Gear | 10 |
Maximum Speed | 400 rpm |
Power | 70 W |
Rollers | 2 |
Tube | 2.4 mm wall lubing |
Delivery Pressure | max. 2bar |
2.4 mm wall tubing | Flow rates
(ml/min at 100 rpm) |
0.5 | 4.1 |
0.8 | 10.5 |
1.6 | 42 |
3.2 | 170 |
4.8 | 380 |
6.4 | 680 |
8.0 | 1040 |
9.6 | 1500 |
Number of mass flow controllers | up to 4 |
Ambient conditions | 5-40 [°C], max. 85 [%rH] |
Input |
up to 4 gases, push in connector 6 [mm] (air, oxygen,nitrogen, carbon dioxide) (gas mixtures can also be connected) |
Input pressure | 1-2 bar |
Output | 1, (tube fitting for tube ID 13 [mm]) |
Flow rate | 0 - 20 [sL/min] |
Accuracy | ±1.5% from the end value |
Pressure sensor |
integrated, 0-400 [mbar], programmable prevents overpressure in the connected vessel |
Air filter | integrated, prevents blocking the mass flow controllers |
Back pressure valve | integrated, prevents reflux into the mass flow controllers |
|
Controlled filter heating box |
for the outlet filters, prevents blocking of the filters caused by condensation (only air inlet and outlet with silicon tubing) |
C-Flex material | for the tubing, enables the user to connect additional components via tube welding |
Technical data subject to change
Cultivate with a 50L single-use bag in an orbital shaken bioreactor SB200-X.
The SB200-X has a cooling ring embedded in the base plate. Cooling is achieved by connecting an external cooling device. An integrated valve, controlled by the Software, regulates the set point temperature.
A tubing support bar is available for attaching the various tubes (feeding, medium, air and pH regulation) and keeping them tidy.
IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.
The gamma irradiated bag for the Kuhner shaken bioreactor requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. The single-use bag has a working volume from 50 litres up to 200 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
Order number | Description |
|---|---|
105331 | Bag for SB200-X with optical sensors |
Order number | Description |
|---|---|
107621 | Red
overpressure valve, OD 14mm |
106490 | Glass
fibers for pH/DO probes, length 1.5m |
The stainless steel adapter enables to use a 50L single-use bag in the orbital shaken bioreactor SB200-X. The stainless steel adapter and 50L single-use bag can easily be installed and operated in the orbital shaken bioreactor SB200-X. This option gives the flexibility to cultivate with either a 50L or a 200L single-use bag in the same orbital shaken bioreactor.
Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces. More.
Fluid flow and mixing dynamics in a shaken bioreactor
Orbital Mixing as an Alternative to Stirred-Tank Bioreactors
Scale-up Production of Pharmaceutical Proteins in Plant Cell Suspensions
Orbital Shaken Bioreactor for Cell Cultivation
Orbital shaken bioreactors in the field of cell cultivation
Characterization of orbitally shaken single-use bioreactors
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
Yes, no other equipment is required.
No, the single-use bag is simply placed in the SB200-X vessel, positioned and then inflated.
No, shaken systems have a very homogenous and low local power input. This means that high local power inputs which are responsible for mechanical stress, simply don’t occur.
Yes
Yes
Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.
Adolf Kühner AG (Headquarters)
Dinkelbergstrasse 1
CH-4127 Birsfelden (Basel)
Switzerland
We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
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