Feeding Technology for Fed-Batch
Are you searching for the most productive strain for a fed-batch process?
The Kuhner Feeding Technology provides an innovative tool for efficient high-throughput screenings in fed-batch mode. The diffusion-driven fed-batch system is delivered sterile and ready-to-use for the instant application in the lab. The Kuhner Shaker GmbH offers three items applying the Kuhner Feeding Technology: Feed Plate® , FeedBeads® and FeedTubes®.
By using the fed-batch mode instead of batch cultivations, overflow metabolism with undesired side-product formation can be significantly reduced and oxygen limited conditions can be avoided. Thereby, screening results are improved and a higher product formation is achieved.
Superior outcome in screening
For efficient scale-up
Reproducible results
Application
Further information
Cultivation conditions decide about which strain is chosen as the best
Using the Kuhner Feeding Technology, cultivation conditions of the strain screenings are more comparable to those on a production scale, since overflow metabolism, pH inhibition, and oxygen limitation are prevented. Thus, an unfavorable strain selection is avoided already early on. Comparable and reproducible conditions in screening allow you to identify the optimal production strain already at an early time point. Consequently, this reduces costs as well as time-consuming process adaptations during later stages in process development.
The Benefits of Feeding Technology for Your Screening
Universal tool
for MTP, shake QWflasks and tubes
Various substrates and release rates
for individual needs
Fast and easily integrated
sterile and ready-to-use
Reducing negative influences
O2-limitation, by-products and overflow, pH-shifts
Improving Screening
controlled cell metabolism and higher significance of results
Simplified scale-up
starting process development one step earlier
Selection of the best strain
for later production process
High product yields
improved cell metabolism
extended cultivation time
Reduction of costs
avoidance of unforeseen development costs
Better screening results
FeedPlates®
Product Portfolio Feeding Technology
Our feeding products (Feed Plate®, Feed Bead®, Feed Tubes® & specialty products) are supplied either as standard stock items or manufactured on request (on demand) according to customer specifications.
Standard products are generally available and are typically shipped within 1–2 weeks.
On-demand products are marked as such and are produced after your order. The typical lead time is approximately 6–8 weeks. The minimum order quantity is 10 Feed Plates® or 10 Feed Beads® (Feed Tubes®: 25 units).
Individual requirements can be discussed at any time. Please feel free to contact us via fdngdkhnrcm or ffcdkhnrcm.
Glucose
Low, Standard and High release
| Type | Article | Substrate | Release after 48 h * | Status |
|---|---|---|---|---|
| 96 well, round | SMFP00701 | Glucose | 3 mg | on demand |
| 96 well, round | SMFP01001 | Glucose | 5 mg | on demand |
| 96 well, round | SMFP08001 | Glucose | 10 mg | on demand |
| 96 well, round | SMFP02701 | Glucose | 15 mg | on demand |
| 96 well, square | SMFP00702 | Glucose | 4 mg | |
| 96 well, square | SMFP01002 | Glucose | 6 mg | on demand |
| 96 well, square | SMFP02002 | Glucose | 9 mg | |
| 96 well, square | SMFP04002 | Glucose | 12 mg | on demand |
| 96 well, square | SMFP08002 | Glucose | 14 mg | |
| 96 well, square | SMFP02702 | Glucose | 18 mg | |
| 24 well, square | SMFP00703 | Glucose | 18 mg | |
| 24 well, square | SMFP01003 | Glucose | 27 mg | on demand |
| 24 well, square | SMFP02003 | Glucose | 40 mg | on demand |
| 24 well, square | SMFP04003 | Glucose | 50 mg | on demand |
| 24 well, square | SMFP08003 | Glucose | 60 mg | |
| 24 well, square | SMFP02703 | Glucose | 80 mg | |
| 48 well, round | SMFP08004 | Glucose | 23 mg | on demand |
| 48 well, round | SMFP02704 | Glucose | 36 mg | on demand |
Glycerol
Low and High release
| Type | Article | Substrate | Release after 48 h * | Status |
|---|---|---|---|---|
| 96 well, round | SMFP11001 | Glycerol | 4 mg | on demand |
| 96 well, round | SMFP12001 | Glycerol | 7 mg | on demand |
| 96 well, square | SMFP11002 | Glycerol | 6 mg | |
| 96 well, square | SMFP12002 | Glycerol | 9 mg | |
| 24 well, square | SMFP11003 | Glycerol | 25 mg | |
| 24 well, square | SMFP12003 | Glycerol | 40 mg | |
| 48 well, round | SMFP11004 | Glycerol | 10 mg | on demand |
| 48 well, round | SMFP12004 | Glycerol | 16 mg | on demand |
Sucrose
| Type | Article | Substrate | Release after 48 h * | Status |
|---|---|---|---|---|
| 96 well, square | SMFP02702 | Sucrose | 7 mg | on demand |
Dummy (no substrate)
| Type | Article | Substrate | Release after 48 h * |
|---|---|---|---|
| 96 well, round | SMFP00001 | no substrate | no release |
| 96 well, square | SMFP00002 | no substrate | no release |
| 24 well, square | SMFP00003 | no substrate | no release |
* Release determined at 37 ° C in 10 mM PBS buffer, pH 7.4
For your high-throughput screening, we recommend that you check the substrate release under your own cultivation conditions and with your own media.
For samples, orders or a personal consultation contact us!
FeedBeads®
Easy application of fed-batch in shake flasks
Product Portfolio Feeding Technology
Our feeding products (Feed Plate®, Feed Bead®, Feed Tubes® & specialty products) are supplied either as standard stock items or manufactured on request (on demand) according to customer specifications.
Standard products are generally available and are typically shipped within 1–2 weeks.
On-demand products are marked as such and are produced after your order. The typical lead time is approximately 6–8 weeks. The minimum order quantity is 10 Feed Plates® or 10 Feed Beads® (Feed Tubes®: 25 units).
Individual requirements can be discussed at any time. Please feel free to contact us via fdngdkhnrcm or ffcdkhnrcm.
Packing size:
Ø 12 mm: 25 beads / pack
Ø 24 mm: 20 beads / pack
Glucose
| Article | Bead size | Substrate | Release after 48 h * | Status |
|---|---|---|---|---|
| SMFB01001 | Ø 12 mm, h= 3 mm | Glucose | 32 mg | on demand |
| SMFB08001 | Ø 12 mm, h= 3 mm | Glucose | 75 mg | |
| SMFB08002** | Ø 24 mm, h= 3 mm | Glucose | 220 mg | |
| **packaging size: 20 beads/pack | ||||
Glutamine
| Article | Bead size | Substrate | Release after 48 h * | Status |
|---|---|---|---|---|
| SMFB78011 | Ø 12 mm, h= 3 mm | Glutamine | 15 mg | on demand |
* Release determined at 37 ° C in 10 mM PBS buffer, pH 7.4
For your high-throughput screening, we recommend that you check the substrate release under your own cultivation conditions and with your own media.
Glycerol
| Article | Bead size | Substrate | Release after 48 h * |
|---|---|---|---|
| SMFB11001 | Ø 12 mm, h= 3 mm | Glycerol | 28 mg |
| SMFB12001 | Ø 12 mm, h= 3 mm | Glycerol | 40 mg |
Sucrose
| Article | Bead size | Substrate | Release after 48 h * | Status |
|---|---|---|---|---|
| SMFB38021 | Ø 12 mm, h= 3 mm | Sucrose | 60 mg | on demand |
| SMFB38022 | Ø 24 mm, h= 3 mm | Sucrose | 150 mg | on demand |
Dummy (no substrate)
| Article | Bead size | Substrate | Release after 48 h * |
|---|---|---|---|
| SMFB00001 | Ø 12 mm, h= 3 mm | no substrate | no release |
For samples, orders or a personal consultation contact us!
Kuhner Notes
Poster
Kuhner FeedingTechnology
Fed-Batch Micro Plates
Fed-batch Mode in Shake Flasks
Simple shake flask cultivation of Komagataella phaffii with constant glycerol feed
Publications
- Small‑scale fed‑batch cultivations of Vibrio natriegens: overcoming challenges for early process development (Lüchtrath et al. Bioprocess and Biosystems Engineering, 2025)
- Polymer-based controlled-releasefed-batch microtiter plate – diminishing the gap between early process development and production conditions
(Keil et al. Journal of Biological Engineering, 2019)
- Precultures Grown under Fed‐Batch Conditions Increase the Reliability and Reproducibility of High‐Throughput Screening Results
(Keil et al. Biotechnology Journal, 2019)
- Rewiring carbon metabolism in yeast for high level production of aromatic chemicals (Nature communications, 2019)
- Fed-Batch Mode in Shake Flasks bySlow-Release Technique
(Biotechnology and Bioengineering, 10/2006)
In our Science Room, we provide general information and data on cultivation processes in Shaken bioreactors.
Feeding Technology - Application of the Kuhner FeedPlate®
Learn how to perform a fed-batch in a microtiter plate.
Discover more about Feeding Technology on our website: https://www.feedingtechnology.com
Good to know
What does the FeedPlate® do?
The FeedPlate® allows the simulation of a Fed-Batch cultivation on a very small scale. Using the FeedPlate®, you can easily obtain reliable statements how your strains behave under Fed Batch conditions in the screening process.
What is the FeedPlate®?
The FeedPlate® consists of a commercially available microtiter plate with our special polymer matrix at the bottom of each well immobilized. This matrix contains the substrate.
How does the FeedPlate® work?
The FeedPlate® is a polymer-based release system. After the microtiter plate comes into contact with culture medium, the release of the substrate starts according to an evendefined rate over a period of up to 7 days.
Do I need special media to work with the Feed Plate®?
No. The Feed Plate® is a physicochemical system and does not require enzymes or special media or additives. The release kinetics is mainly dependent on the temperature and osmolarity of the medium used. Therefore, we refer to the release rate in the product catalog to a reference medium or cultivation conditions.
How many samples can I process with the FeedPlate®?
The FeedPlate® is currently available as standard in 24-well, 48-well and 96-well format, which allows you to process many samples in parallel in a reliable manner. This allows you to screen easily and quickly in high throughput.
What is the format of the FeedPlate®?
The FeedPlate® is available in commercially available microtiter plate formats. You choose which format you want to use. We produce special formats on request.
However, I only use microtiter plates from a certain manufacturer.
No problem, we can theoretically dose our matrix into any type of microtiter plate (cell culture tubes, 6-, 12-, 24-, 48- or 96-well MTPs), also different materials (PP, PS, etc.) or plates from certain manufacturers are possible (on request).
Are substrates other than C sources possible?
In addition to the standard carbon source glucose, we also offer other substrates such as maltose and fructose, and complex media such as yeast extract or other substrate classes such as nitrogen and phophate are also possible. Please contact us!
I need a high OTR (oxygen transfer rate). Can I still work in the 96 Deepwell plate?
Yes, that is not a problem. As long as you cultivate in Fed-Batch mode, oxygen supply should not be a problem. If you have any questions about how to reach a specific OTR, we will be happy to advise you and provide appropriate literature.
How should the FeedPlate® be stored?
The FeedPlate® should be stored dry at room temperature. Direct sunlight should be avoided.
How stable is the FeedPlate®?
The substrates are usually incorporated into the matrix as stable crystalline substances. Shelf life is therefore a question of sterility. The Feed Plate® is sterilized by beta radiation and is at least durable for one year.
You have a question that we do not answer here?
Contact us: Call us or write us an e-mail. We will be happy to answer your open questions.
Our flyers are currently being updated.
Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.
Contact
Adolf Kühner AG (Headquarters)
Dinkelbergstrasse 1
CH-4127 Birsfelden (Basel)
Switzerland
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