For instance, there is no need for (bolus) feeding of the cells on weekends, since the feeding is done by the FeedBeads. Another great advantage is the continuous feeding of substrate (fed-batch) by the FeedBeads, in contrast to the usually performed batch cultivations with subsequent bolus feeding.

Mammalian cell cultivations that start as batch cultivations with high nutrient concentrations and receive subsequent bolus feeding can alter in their metabolic profiles. These conditions yield an inefficient cell metabolism and have been linked to increased concentrations of unwanted by-products (e.g., lactate or ammonia), which in turn have been shown to limit cell growth and productivity in mammalian cell processes. By maintaining a low concentration of substrate over time, e.g., in a fed-batch process with FeedBeads, these metabolic inefficiencies can be avoided and the productivity of a process improved.
In our recent application note, the influence of glutamine releasing FeedBeads on the growth and productivity of CHO cells is reviewed. A detailed protocol of application is presented, and the performance of the FeedBeads for different filling volumes are discussed. The results demonstrate that the glutamine releasing FeedBeads can be used to optimize a CHO cultivation process and that the filling volume will impact the release kinetics and yield. You can find this AppNote and many more in our Science Room in the section Kuhner Notes.

If you are interested in the Feeding Technology or have any questions, please visit our website or contact us via deoffice@kuhner.com.