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Determine the maximum oxygen supply!

What is the impact of medium, shaking speed, shaking diameter and flask size?

Flask
shake flask diameter [mm]
i
filling volume [ml]
i
shaking diameter [mm]
i
media OSMO [osmol/kg]
i
pressure [bar]
i
O2 concentration
i
1
2
3

For cell culture applications, please refer to the additional information below the graph.

Maximum oxygen transfer capacity [mmol/l/h] *

Kuhner shaker
Kuhner shaker

Additional information for cell culture applications

If your specific cell culture medium is not available in the “media OSMO” options, a good general guideline is to use 301 osmol/kg. The variation in osmolarity across different cell culture media is typically small, making this a reliable average.

When using CO₂ in your incubator, it is important to check the advanced settings and enter the correct new oxygen concentration.

New oxygen concentration:

  • 5% CO₂ atmosphere → Enter 0.199% O2 concentration
  • 8% CO₂ atmosphere → Enter 0.193%O2 concentration

For other concentrations or any questions, feel free to contact us at shakingtechnology@kuhner.com

* Calculated values: Meier K. et al, Correlation for the maximum oxygen transfer capacity in shake flasks for a wide range of conditions and for different culture media, Biochemical Engineering Journal, 2016 All information is subject to change.


Check out the formula and definitions of parameters

Kuhner Notes: Maximum oxygen transfer capacity