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LT-X incubator shaker

LT-X incubator shaker
Overview
Overview
Technical Data
Options
Accessories
Science
FAQ
PDF

Space saving stackable incubator shaker

The Kuhner LT-X (Lab-Therm) incubator shaker has the same capabilities as the larger ISF1-Z and ISF4-X models. The incubator shaker was specially developed to address the shaking needs of the biotechnology and pharmaceutical industries. The practical size of the LT-X means it will fit in any laboratory.

Performance characteristics

  • Fits into any laboratory 

  • Used in biotech and pharmaceutical industries

  • Accepts flasks up to 6 litres

  • Two units can be stacked without the need for special kits or tools

  • Humidity, CO2 and O2 control available

5 year warranty

5 year warranty

CO2 control

CO2 control

O2 control

Ocontrol

Humidity control

Humidity control

Further information

Shaking diameter

Dr. Shakers Helpful Hint N°4 explains what the shaking diameter is and how you can determine the shaking diameter of your lab shaker.

Dr. Shaker

Find information on the importance of the shaking diameter for biological cultivations in our Shaking Technology Forum: Why is the shaking diameter important?


Extra space

Extra space

Exceptional chamber height means flasks up to 6 litres capacity can be accommodated. If using smaller flasks, there is sufficient head height to fit one or two stationary trays above the shaking table. It is also possible to stack two LT-X together without the need for special kits or tools.

LT-X gestapelt

Direct drive

Functionality through direct drive

Direktantrieb

The maintenance-free direct drive guarantees long and economical performance from the LT-X. The shaking diameter can be changed at any time thanks to Kuhner’s unique direct drive system. The integrated drive is protected from spills and the all stainless steel interior has a drain at the back for any unwanted liquids. The chamber can be cleaned using chemicals or sterilized with optional UV lighting.


Safety

Safety and quality 

A door switch ensures the motor stops as soon as the door is opened. After closing the door properly, the motor restarts. The safety, reliability and quality of our shakers are of personal concern to everyone at Kuhner which is why all our machines are supplied with a 5 year warranty. 

We subject all our shaking machines to an elaborate final test before delivery and send the test protocols to our customers.


Configuration

Configuration

The LT-X can be equipped with options for CO2, O2,controlled humidity, LED photosynthesis, UV lighting and much more. It is now also possible to integrate measuring data from Kuhner shakers with your company’s own network using Kuhner Insight software together with the NET-60 interface.

Technical Data 

Baseline model

LT-X Basic

LT-X EcoDew®

LT-X Compressor

108672

108672 + 102011

108672 + 102008

Baseline model with CO₂ control (optimized for cell culture)

LT-XC Basic

LT-XC EcoDew®

LT-XC Compressor

108671

108671 + 102011

108671 + 102008

+ Option Humidity

LT-X(C) Basic

LT-X(C) EcoDew®

LT-X(C) Compressor

not available

included

102010

+ Option O₂

LT-X(C) Basic

LT-X(C) EcoDew®

LT-X(C) Compressor

102012

102012

102012

Technical Overview


LT-X(C) Basic

LT-X(C) EcoDew®

LT-X(C) Compressor

Cooling

no

no

yes

Humidity control

no

yes

no / yes

Temperatur minimal

ambient + 10 °C

ambient + 10 °C

ambient – 15 °C (– 10 °C)*

Temperatur maximal

80°C (60°C)*

80°C (60°C)*

80°C (60°C)*

Humidity maximal

-

85 % r.h.

- / 85 % r.h.

Power consumption

< 700W

< 1050W

< 880W / < 1150W

* Shaking incubator optimized for cell cultures

Technical changes reserved

Machine

Gas volume 

260 l

Weight (with cooling)

170 kg

Illumination

LED

Operating menu in

German, French, English, Italian, Spanish 

Interface, standard

CAN-Bus

Interface, optional

USB, Ethernet, digital, analogue

Ambient temperature

10°C up to 35°C

Dimensions (in mm)

Left sidewall

Cable feedthrough: PG 16 (ID 20mm)

Optional: 2nd cable feedthrough 

Temperature

Setting, digital

0.1°C

Accuracy, absolute

± 0.30°C (37°C)
± 0.25°C (37°C)*

Principle of sensor

Pt-100

Power of heating

500 W

Power of cooling

90...155 W

Air circulation

160 m3/h

Humidity

max. at 25..55°C

85% r.h.

Setting, digital

1% r.h.

Accuracy, absolute

± 2% r.h.

Principle of sensor

capacitive

Water refill

automatic

Water heater

180 W

Door heater

90 W

CO₂

Principle of sensor

Infrared, NDIR

Measuring range

0...20% CO2

Setting, digital

0.1%

Accuracy, absolute
(including non-linearity, calibration uncertainty, repeatability)

± 0.40% at 5% CO2

Temperature range

5...60°C

CO2-supply

max. 2 bar overpressure

O₂

O2 sensor

zirconium oxide

Measuring range

approximately 0% up to ambient O2 (20.95% at dry air)

Accuracy, absolute

±0.40% at 5% O2

O2 sensor stability

±0.5% full scale/year

Setting, digital

± 0.1%

O2 control 

PID, digital

Temperature range

-10...80°C

N2-supply

max. 0.5...0.8 bar overpressure

Mains connection

110 - 120 V / 50-60 Hz

95 - 105 V / 50-60 Hz

Shaking unit

Tray size

EX (500 x 420mm)

Loading, maximum

25kg

Setting, digital

1 rpm

Accuracy, absolute

≤ 800 rpm = ± 0.5 rpm
> 800 rpm = ± 1.0 rpm

Timer

1s ... 999h

Acceleration

controlled

Active brake

adjustable

Stop on position

adjustable

Shaking motion


Speed

* orbital, Ø 12.5mm

20...500 rpm

* orbital, Ø 25.0mm

20...400 rpm

* orbital, Ø 50.0mm

20...300 rpm

* linear, ↔ 12.5mm

20...400 rpm

* linear, ↔ 25.0mm

20...300 rpm

* linear, ↔ 50.0 mm

20…200 rpm

* can be changed

Technical data subject to change

Overview Options 

Do you know these options?

A simple and economical way of doubling the shaking capacity.

EPFL-Tisch

Touchscreen with Kuhner Insight Software for simple operation, monitoring and programming of incubator shakers.

Icon Touchscreen

Have you heard about our new EcoDew®-Technology? Find more information under kuhner technology.

Icon EcoDew

Read more about our new O2 control option.

Icon automatische Tür

CO₂ control

CO2 control is essential when working with mammalian cell cultures. Kuhner was the first company to manufacture and supply incubator shakers with CO2 control more than 15 years ago and continues to offer this option. More.


O₂ control (PhysOx control)

A reliable control of O2 is essential for cultivating cells (proliferation and metabolism of stem cells) and microorganisms with low/no oxygen demand. The O2 controller reduces the O2 level from ambient (20.95% at dry air; normoxia) to approximately 0% O2 (hypoxic). A digital PID controller adjusts the N2 flow rate to maintain the desired O2 value.

Visit our poster gallery and find more information on the poster "Oxygen Transport Phenomena in Single-Use Shaking Flasks under Reduced Oxygen Conditions".pdf


Cooling

All Kuhner incubator shakers can be fitted with cooling. The refrigeration system is regulated by a fully digital PID control ensuring minimal energy consumption. The cooling is switched on only when required as determined by the chamber temperature and set point. More.


Humidity control

Humidity control is an important factor in successful fermentation. Evaporation from microtiter plates, or when cultivating in flasks for long periods (e.g. cell cultures), can be significantly reduced with humidification.

To reduce evaporation from shake flasks or microtiter plates a water bath is placed inside the incubator. This water bath is fitted with an automatic water supply.

Our newly developed EcoDew®-Technology provides precise humidity control. More.


Illumination unit for photosynthesis (LED)

The ceiling of any Kuhner incubator shaker can be fitted with LED modules for the cultivation of phototrophic organisms. The control module allows full programming of night/day cycles and variable light intensity.

LED

UV lamp

As in a biological safety cabinet, the chamber of a Kuhner incubator shaker can also be sterilized with an integrated UV lamp. The lamp is situated to maximise irradiation of the chamber and shaking table. The UV lamp has a clearly marked external switch and when the door is opened, the UV-lighting is automatically switched off.


Light shade plate

Kuhner has designed a new light shade plate for the LT-X. This plate is easily placed in front of the door window and held in position with clamps. It prevents radiation affecting the contents of the incubator chamber and protects light sensitive media from UV, artificial and/or daylight.

Simply lift the light shade plate to look at the cultures without opening the incubator door. When light protection is no longer required, the plate is easily removed.

Check out the Kuhner EquipNote "Light protection for Kuhner incubators".

Lichtschutzblende

Black window

Available for light sensitive medium or organisms. Any Kuhner incubator shaker can be delivered with blackened windows to prevent unwanted daylight or UV radiation inside the incubator.

Check out the Kuhner EquipNote "Light protection for Kuhner incubators".


Touchscreen

This option includes a 7-inch touchscreen with our Kuhner Insight Software. The touchscreen has been designed for simple operation, monitoring and programming. It can also be retrofitted to existing incubator shakers. More.


Dual table

The dual table is an easy and economical way of doubling the shaking capacity. The dual shaking table consists of two levels. Each level will accept an E (420 x 420 mm) or EX (500 x 420 mm) size tray.

The dual table is available with a fixed distance between the two levels of either 120, 160, 180, or 200 mm. At the maximal distance of 200 mm the lower level accepts 125 mL, 250 mL, and 500 mL Erlenmeyer shake flasks.

However, with the dual table the shaking speed is limited to 200 rpm at a shaking diameter of 50 mm (all other maximum shaking frequencies, dependent on the shaking diameter, can be withdrawn from the LT-X manual).

Ausziehtisch

EPFL-table

Do you perform screenings with a large number of 50 ml bioreactors (TPP, Falcon etc.) or microtiter plates? Would you like to shake different cultivation vessels on the same shaker and be able to change the composition quickly and easily? Then the Kuhner EPFL-table is the optimal solution for you. The shaker table has 5 individual clamps, so that 5 different holders or EPFL universal trays can be clamped. By opening a clamp, a single holder or universal tray can be easily removed from the incubator shaker and processed under a clean bench while the remaining holders/trays keep on shaking.

The EPFL universal tray can be equipped with clamps for Erlenmeyer flasks, Schott laboratory bottles, test tube holders, microtiter plates or customer-specific holders. Rearrangement of these clamps and holders is quick and easy. The universal tray can also be equipped with sticky strips. Sticky strips are used to fix shake flasks or other equipment on shaking trays. They offer easy handling and flexible positioning of different sizes and shapes of shake flasks and other vessels.

Special holders for microtiter plates, 50 mL centrifuge tubes (Falcons) and 600 mL reaction vessels are also available for the EPFL-table.

Instead of 5 individual holders, a large Kuhner F-tray (800 x 420mm) can also be clamped onto the shaker table.

For the smaller (incubator) shakers from Kuhner, correspondingly adapted EPFL-tables with 3 or 4 clamps are available (LT-X: 3 clamps, LS-Z: 3 or 4 clamps).

EPFL

EPFL-table

EPFL

EPFL-table with different holders 

EPFL

F-size tray clamped on the EPFL-table

EPFL

Clamp handle 

Individual parts

Order number

Description

100125

Holder for 24 x 50 ml Falcon/TPP tubes mounted on a base plate

Halterung für 24 x 50 ml Zentrifugenröhrchen
Halterung für 24 x 50 ml Zentrifugenröhrchen

Order number

Description

104173

Cover clamp for 4 deepwell MTPs (Duetz)

Halterung (Duetz) für 4 Deepwell Mikrotiterplatten (MTP)

Order number

Description

104104

Universal clamp for 12-16 lowwell MTPs or 4 deepwell MTPs (Duetz)

Halterung (Duetz) für 12-16 Low-Well MTP oder 4 Deep-Well MTP

Order number

Description

107322

EPFL-Tray Universal kpl.

can be fitted with clamps

EPFL-Tablar Universal kpl.

Order number

Description

105186

Holder 5x600mL TPP tubes, straight tubes

108226

Tube set for holder 5 x TPP 450ml

Reagenzglashalter für 5x600mL TPP tubes

Order number

Description

100633

Holder for 3 x 600ml reactor

Halter für 3 x 600ml Reaktoren

Kuhner TOM

Kuhner TOM (Transfer-Rate Online Measurement) measures online oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR) and the respiratory quotient (RQ) in up to 16 individual shake flasks for better process understanding and informed process development. TOM is easily installed in any Kuhner shaker and can be operated with various flask types and sizes including single use shake flasks. Kuhner TOM has zero footprint on the shaker tray as it is mounted above (see picture below). More.

Kuhner TOM

AerationSystem and FlowCon

AerationSystem and FlowCon provide the ideal system for carrying out cell/stem cell cultivations in a simple, fast, and inexpensive manner.

Lab shakers in temperature-controlled rooms and incubator shakers without humidity and CO2-control can be equipped with the AerationSystem and FlowCon to easily implement humidification and a controlled gas composition for carrying out cell cultivations (e.g., CHO, HEK cells…) in shake flasks.

AerationSystem

The FlowCon is a stand-alone gas mixing device and provides a precise gas composition and flow rate. Simultaneously, the AerationSystem (consisting of GasDivider and special shake flask caps) humidifies and distributes the gas flow evenly to the connected shake flasks. Evaporation losses from the shake flasks are significantly reduced, which is particularly advantageous for very long cultivation times (such as cell culture processes) and contributes to successful cultivations. Another advantage is that opening the incubator door does not affect the gas composition in the shake flasks when they are connected to the aeration system.

By controlling the composition of the inlet air with the FlowCon, it is not only possible to supplement CO2 but also to increase or reduce the concentration of oxygen (O2) or implement anoxic conditions using nitrogen (N2). This control of gas compositions allows for a variety of different applications, including stem cell processes for gene therapy or cultivated meat that require a physiological oxygen concentration at 5% O2 or anaerobic cultivations in a nitrogen atmosphere.

FlowCon

The patented Kuhner GasDivider humidifies the mixed, dry gas flow provided by the FlowCon and distributes it precisely and evenly across the attached shake flasks, independent of shake flask type, tube length and any filters in-between. As the GasDivider is placed on the shaking tray of the (incubator) shaker, the gas flow is also perfectly adapted to the temperature of the incubator shaker or temperature-controlled room.


Perspex cover

To prevent accidental operation of the controllers, the panel can be protected by a perspex cover.


TabCom

The TabCom option from Kuhner consists of a cable for power and data with the connection port integrated in the shaking table (see picture below). A cable guide prevents the cable breaking and ensures secure data recording. The flexibility of TabCom means online measurement systems can be easily integrated.

Port_TabCom

IQ-OQ-Documentation

IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.

Touchscreen

Overview Accessories 

Universal tray

The universal tray can be fitted with clamps for Erlenmeyer flasks, test tube holders, microtiter plates, or custom made holders to suit your requirements. 

Rearranging these clamps and holders is quick and simple.

Order number

Description

102201

Universal tray EU (420 x 420 mm)

102207

Universal tray EXU (500 x 420 mm)

Universaltablar

Tray with fixed clamps

The clamps are riveted onto these trays and cannot be removed. They offer maximum stability and will often hold a larger number of flasks than universal trays. These trays are available in a variety of flask clamp sizes from 25 to 6000ml.

Tray E (420 x 420 mm)

Ordner number

Description

Number of clamps

101525

E-25 ml

81

101526

E-50 ml

50

101527

E-100 ml

39

101528

E-125 ml

30

101529

E-150 ml

30

101530

E-200 ml

20

101531

E-250 ml

18

101532

E-300 ml

15

101533

E-500 ml

12

101534

E-1000 ml

9

101535

E-1500 ml

5

101536

E-2000 ml

5

101537

E-3000 ml

4

101538

E-4000 ml

2

101539

E-5000 ml

2

101540

E-6000 ml

1

Tray EX (500 x 420 mm)

Order number

Description

Number of clamps

101541

EX-25 ml

90

101542

EX-50 ml

60

101543

EX-100 ml

42

101544

EX-125 ml

36

101545

EX-150 ml

32

101546

EX-200 ml

25

101547

EX-250 ml

21

101548

EX-300 ml

18

101549

EX-500 ml

14

101550

EX-1000 ml

9

101551

EX-1500 ml

8

101552

EX-2000 ml

5

101553

EX-3000 ml

4

101554

EX-4000 ml

3

101555

EX-5000 ml

3

101556

EX-6000 ml

2

F_Tablar_mit_genieteten_Klammern_Kuhner

Tray for microtiter plates 

Kuhner has designed trays specifically for microtiter plates. Handles at the front of the tray open and close the special clamping mechanism allowing plates to be loaded and unloaded. Trays of different heights (22, 47 and 77mm) allow the stacking of up to four microtiter plates. The trays are available in three sizes and suitable for all types of plates (deep well, low well, 24, 48 and 96 well).

Order number

Description

Numbers of clamps

104947

E-MT.22

12 to 24

104950

E-MT.47

12 to 48

104948

E-MT.77

12 to 72

Tray_Microtiterplates

Tray for microtiter plates (springs)

Kuhner has designed a new tray for microtiter plates. This tray is fitted with springs which allows each well plate to be loaded and unloaded individually. The tray is available in three sizes: E (420x420mm), EX (500x420mm) and F (800x420mm).


This new tray is particularly suitable for the special holder with filter lid for deep well microtiter plates.

Order number

Description

Number of mircotiter plates

104806

E-tray(420 x 420 mm) for deepwell or mircrotiter plates

1-12

104794

EX-tray (500 x 420 mm) for deepwell or mircotiter plates

1-15

F-Tablar für Mikrotiterplatten (Federn)

Tray with rubber mat

The whole tray is covered with a rubber mat and is particularly suitable for shaking containers at low speed (e.g. blood bags).

Order number

Description

105312

Rubber mat  EG
(420 x 420 mm)

105313

Rubber mat EXG
(500 x 420 mm)

Tablar mit Gummimatte

Tray with sticky strips 

A tray fitted with sticky strips is especially useful for flasks shaken at low speeds (up to 200 rpm). The flask is placed directly on the sticky strip without the need for any other support. Flasks of all sizes can be used. Sticky strips are available individually or in sets and can also be supplied already fitted to a tray. The EX-size tray accepts five strips. 

Order number

Description

105169

E-tray with 4 sticky strips (clear)

105199

EX-tray with 5 sticky strips (clear)

105194

1 sticky strip: 385 x 85 x 3 mm (clear)

Tablar mit Klebestreifen

Tray with PC-plate for sticky strips

The PC-plate increases the adhesion of the sticky strips. This is particularly helpful if using flasks >500ml.

Order number

Description

105224

EX-tray with 5 sticky strips (clear)

105194

1 sticky strip: 385 x 85 x 3 mm (clear)

105196

1 big sticky strip: 395 x 385 x 3 mm (clear)

Tray with PC-plate for sticky strips

Dual tray

The dual tray offers two shaking levels and doubles the capacity. It is available in EXU, EX, FU and F size and the upper tray can be removed.
Please note the shaking speed is limited.

Order number

Description

100548

two EXU trays with 200mm distance (with holes)

102226

two EX trays with 200mm distance
(no holes for sticky strips /rubber mats)

Doppeltablar

Tray with support bars

These trays offer an alternative to both clamps and sticky mats and are ideal for tall containers. The tray is covered with a rubber mat and has an upright at each corner. The EX size tray is fitted with four cross supports. These support bars securely clamp containers.

Order number

Description

Number of longitudinal girders

106408

EA-tray with rubber mat and 4 cross supports  

2

106407

EXA- tray with rubber mat and 4 cross supports 

2

F-Tablar mit Aufbausystem und Querträger

Tray for separating vessels

Kuhner has also designed a special tray for shaking separating vessels. This very flexible tray can take vessels of various sizes. It is also possible to add a second layer and double the capacity. Movable retaining rods allow vessels to be installed easily.

Order number

Description

Number of separating vessels

SM420250

ES-250

5

SM4202502

ES-250/2

10 (2 layers)

SM420500

ES-500

4

SM4205002

ES-500/2

8 (2 layers)

SM421000

ES-1000

3

SM4210002

ES-1000/2

6 (2 layers)

Tablare für Scheidetrichter

Floor stand

For a comfortable working height Kuhner offers floor stands for both the ISF1-Z and LT-X incubator shakers. These are available in a choice of 400mm (2 x LT-X) or 765mm (1 x LT-X) high.

    Order number

    Description

    100834

    400mm high for 2 x LT-X

    100838

    765mm high for 1 x LT-X

    Floor stand

    Floor stands with wheels

    Floor stands with wheels are available for both the ISF1-Z and LT-X incubator shakers. These stands enable the user to move the machines easily. However, the shaking speed is limited.

    Order number

    Description

    100842

    400mm high for 2 x LT-X

    100841

    765mm high for 1 x LT-X

    105008

    Upgrade costs for castors (Polyamid)*

    *only for new machines

    Untergestell mit Rollen

    Stacking adapter

    This stacking adapter is designed for stacking a LT-X on an ISF1-X/Z incubator shaker. Extra work space on the adapter allows room for flasks or a small machine, as well as the LT-X, thus creating a greater working area.

    Order number

    Description

    105864

    Stacking adapter (LT-X on ISF1-X/Z)

    Stapeladapter

    Kuhner Insight Software

    The user-friendly Kuhner Insight software was developed for supervision, monitoring, and recording of data. The software is easy to operate. More.

    Kuhner Insight Software

    Interfaces

    Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces. More.

    Kuhner Insight Software

    Shelf

    The Kuhner LT-X incubator shaker can be fitted with a shelf allowing cultivation in petri dishes. One or two shelves can be placed above the shaking table and fixed to the chamber walls with four screws.

    Order number

    Description

    102017

    Shelf for LT-X


    Water bath

    To reduce evaporation from shake flasks or microtitre plates a stainless steel water bath can be placed inside the incubator. This water bath is not fitted with an automatic water supply and must be topped up manually.

    Order number

    Description

    100799

    Humidity bath, coated, for LT-X

    Feuchtewanne

    Light shade plate

    Kuhner has designed a new light shade plate for the LT-X. This plate is easily placed in front of the door window and held in position with clamps. It prevents radiation affecting the contents of the incubator chamber and protects light sensitive media from UV, artificial and/or daylight.

    Simply lift the light shade plate to look at the cultures without opening the incubator door. When light protection is no longer required, the plate is easily removed.

    Check out the Kuhner EquipNote "Light protection for Kuhner incubators".

    Order number

    Description

    100855

    Light shade plate for LT-X

    Lichtschutzblende

    Publications


    Kuhner Notes


    Kuhner Notes

    Monitoring of pH and OTR using a multiple shake flask  platform

    Characterization of Algal Cell Culture in Photobioreactors

    Oxygen Transport Phenomena in Single-Use Shaking Flasks


    In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.

    Good to know

    Is linear shaking available?

    Yes

    Is it possible to stack three LT-X together?

    No, only two.

    Is it possible to stack a LT-X on an ISF1-Z? 

    Yes, using a special fitting.

    Is it possible to shake a 6 litre Erlenmeyer flask inside the LT-X?

    Yes, an EX-tray has space for two 6 litre Erlenmeyer flasks.

    Is IQ-OQ documentation available for the shakers?

    Yes

    Can the machine be used in the GMP sector?

    Yes

    The following flyers provide information about the machines and function-specific solutions.

    Kuhner shakers are suitable for the cultivation of microbial, plant, human and animal cell cultures. Find more information about the different application areas and suitable product recommendations for optimal cultivation here.

    Stem cells
    Cell culture
    Bacteria
    Plant cells & algae

    Contact

    Adolf Kühner AG (Headquarters)

    Dinkelbergstrasse 1

    CH-4127 Birsfelden (Basel)

    Switzerland

    Services

    We support our customers in implementation, maintenance and repair of all Kuhner products – efficiently and competently. 

    GMP compliant production of shakers and bioreactors

    For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.

    Read more

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    The world's most reliable shaking machines built in-house in Switzerland.

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