The Kuhner TOM (Transfer-Rate Online Measurement) provides online measurements of the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) for up to 16 individual shake flasks in a Kuhner incubator. The TOM provides the user with a better understanding of any bioprocess and allows for more efficient and effective optimization during process development. It is easily installed in any Kuhner shaker and is compatible with a variety of different shake flask types and sizes, including single use shake flasks.
The modularity of the Kuhner TOM allows the user to choose online measurements from 4, 8, 12, or 16 shake flasks. The TOM itself is mounted above the shake flasks and requires no space on the shaking platform, maximizing the capacity for shake flasks in the incubator.
The Kuhner TOM captures the progress of multiple cultivations over time, providing an opportunity for the user to selectively schedule off-line sampling and analysis at critical time points. The TOM offers valuable insight on the viability and growth rate of shaken cultivations and will also help identify sub-optimal conditions, including oxygen limitation, substrate limitation, or product inhibition. The TOM also provides an opportunity for the systematic and rapid optimization of culture media and conditions (e.g. balancing different carbon sources or media additives, substrate uptake and product formation via RQ). The OTR, CTR, and RQ data captured from the TOM will accelerate any process development and facilitates scale-up.
< 600 rpm
Orbital shaking diameter
3 mm – 70 mm
250 mL – 1000 mL (smaller and larger
flasks on request)
> 4% of nominal flask volume
< 85% rel. humidity
CO2 < 16%
CO2 sensor calibration once per month
Pump adjustment after 1 year
Technical data subject to change
In our Science Room, we offer general information and data of cultivation processes in shaken bioreactors.
For Kuhner, the quality and durability of their shakers and bioreactors have always been the topics with the highest priority. On this account, Kuhner products have also proven their worth worldwide under GMP requirements. Documentation, development and production along the full life cycle of our products are continuously enhanced to match the requirements of 21 CFR and GAMP 5.
The world's most reliable shaking machines built in-house in Switzerland.